Identification of three related human GRO genes encoding cytokine functions

Journal Article · Mon Oct 01 04:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.19.7732· OSTI ID:5681984

Haskill, S; Peace, A; Morris, J; Sporn, S A ^[1]; Anisowicz, A; Lee, S W; Sager, R ^[2]; Smith, T ^[3]; Martin, G; Ralph, P ^[4]

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1. Univ. of North Carolina at Chapel Hill, (USA)
2. Dana-Farber Cancer Institute, Boston, MA (USA)
3. Harvard School of Public Health, Boston, MA (USA)
4. Cetus Corporation, Emeryville, CA (USA)

The product of the human GRO gene is a cytokine with inflammatory and growth-regulatory properties; GRO is also called MGSA for melanoma growth-stimulatory activity. The authors have identified two additional genes, GRO{beta} and GRO{gamma}, that share 90{percent} and 86{percent} identity at the deduced amino acid level with the original GRO{alpha} isolate. One amino acid substitution of proline in GRO{alpha} by leucine in GRO{beta} and GRO{gamma} leads to a large predicted change in protein conformation. Significant differences also exist in the 3' untranslated region, including different numbers of ATTTA repeats associated with mRNA instability. A 122-base-pair region in the 3' region is conserved among the three GRO genes, and a part of it is also conserved in the Chinese hamster genome, suggesting a role in regulation. DNA hybridization with oligonucleotide probes and partial sequence analysis of the genomic clones confirm that the three forms are derived from related but different genes. Only one chromosomal locus has been identified, at 4q21, by using a GRO{alpha} cDNA clone that hybridized to all three genes. Expression studies reveal tissue-specific regulation as well as regulation by specific inducing agents, including interleukin 1, tumor necrosis factor, phorbol 12-myristate 13-acetate, and lipopolysaccharide.

• OSTI ID: 5681984
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:19; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL CELLS
CARBOHYDRATES
CARCINOGENS
CONNECTIVE TISSUE CELLS
DISEASES
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
ESTERS
FIBROBLASTS
GENES
GENETIC MAPPING
GROWTH FACTORS
HYBRIDIZATION
INFLAMMATION
LIPIDS
LIPOPOLYSACCHARIDES
LYMPHOKINES
MAPPING
MELANOMAS
MITOGENS
MOLECULAR STRUCTURE
NEOPLASMS
NUCLEIC ACIDS
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PHORBOL ESTERS
POLYSACCHARIDES
PROTEINS
RECOMBINANT DNA
SACCHARIDES
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
SYMPTOMS