Sequence heterogeneity, multiplicity, and genomic organization of. cap alpha. - and. beta. -tubulin genes in Sea Urchins

Journal Article · Tue Dec 01 04:00:00 EST 1981 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.1.12.1125· OSTI ID:5880541

Alexandraki, D; Ruderman, J V

The authors analyzed the multiplicity, heterogeneity, and organization of the genes encoding the ..cap alpha.. and ..beta.. tubulins in the sea urchin Lytechinus pictus by using cloned complementary deoxyribonucleic acid (cDNA) and genomic tubulin sequences. cDNA clones were constructed by using immature spermatogenic testis polyadenylic acid-containing ribonucleic acid as a template. ..cap alpha.. and ..beta..-tubulin clones were identified by hybrid selection and in vitro translation of the corresponding messenger ribonucleic acids, followed by immunoprecipitation and two-dimensional gel electrophoresis of the translation products. The ..cap alpha.. cDNA clone contains a sequence that encodes the 48 C-terminal amino acids of ..cap alpha.. tubulin and 104 base pairs of the 3' nontranslated portion of the messenger ribonucleic acid. The ..beta.. cDNA insertion contains the coding sequence for the 100 C-terminal amino acids of ..beta.. tubulin and 83 base pairs of the 3' noncoding sequence. Hybrid selections performed at different criteria demonstrated the presence of several heterogeneous, closely related tubulin messenger ribonucleic acids, suggesting the existence of heterogeneous ..cap alpha..- and ..beta..-tubulin genes. Hybridization analyses indicated that there are at least 9 to 13 sequences for each of the two tubulin gene families per haploid genome. Hybridization of the cDNA probes to both total genomic DNA and cloned germline DNA fragments gave no evidence for close physical linkage of ..cap alpha..-tubulin genes with ..beta..-tubulin genes at the DNA level. In contrast, these experiments indicated that some genes within the same family are clustered.

• Research Organization: Dept. of Anatomy and Dept. of Cell and Developmental Biology, Harvard Medical School, Boston, MA 02115
• OSTI ID: 5880541
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 1:12; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL VARIABILITY
CELL CONSTITUENTS
CLASSIFICATION
CLONING
DNA SEQUENCING
DNA-CLONING
ECHINODERMS
ELECTROPHORESIS
ENZYMES
ESTERASES
GAMETOGENESIS
GENE REGULATION
GENES
GENETIC ENGINEERING
GENETIC MAPPING
GENETIC VARIABILITY
HAPLOIDY
HYBRIDIZATION
HYDROLASES
INVERTEBRATES
MAPPING
MESSENGER-RNA
MICROTUBULES
MOLECULAR BIOLOGY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PLOIDY
PRECIPITATION
PROTEINS
RNA
RNA-ASE
SEA URCHINS
SEPARATION PROCESSES
SPERMATOGENESIS
STRUCTURAL CHEMICAL ANALYSIS
TWO-DIMENSIONAL ELECTROPHORESIS