Three human alcohol dehydrogenase subunits: cDNA structure and molecular and evolutionary divergence

Journal Article · Sat Feb 01 04:00:00 EST 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.83.3.634· OSTI ID:6411127

Ikuta, T; Szeto, S; Yoshida, A

Class I human alcohol dehydrogenase (ADH; alcohol:NAD/sup +/ oxidoreductase, EC 1.1.1.1) consists of several homo- and heterodimers of ..cap alpha.., ..beta.., and ..gamma.. subunits that are governed by the ADH1, ADH2, and ADH3 loci. The authors previously cloned a full length of cDNA for the ..beta.. subunit, and the complete sequence of 374 amino acid residues was established. cDNAs for the ..cap alpha.. and ..gamma.. subunits were cloned and characterized. A human liver cDNA library, constructed in phage lambdagt11, was screened by using a synthetic oligonucleotide probe that was matched to the ..gamma.. but not to the ..beta.. sequence. Clone pUCADH..gamma..21 and clone pUCADH..cap alpha..15L differed from ..beta.. cDNA with respect to restriction sites and hybridization with the nucleotide probe. Clone pUCADH..gamma..21 contained an insertion of 1.5 kilobase pairs (kbp) and encodes 374 amino acid residues compatible with the reported amino acid sequence of the ..gamma.. subunit. Clone pUCADH..cap alpha..15L contained an insertion of 2.4 kbp and included nucleotide sequences that encode 374 amino acid residues for another subunit, the ..gamma.. subunit. In addition, this clone contained the sequences that encode the COOH-terminal part of the ..beta.. subunit at its extended 5' region. The amino acid sequences and coding regions of the cDNAs of the three subunits are very similar. A high degree of resemblance is observed also in their 3' noncoding regions. However, distinctive differences exist in the vicinity of the Zn-binding cysteine residue at position 46. Based on the cDNA sequences and the deduced amino acid sequences of the three subunits, their structural and evolutionary relationships are discussed.

• Research Organization: Beckman Research Institute of the City of Hope, Duarte, CA
• OSTI ID: 6411127
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 83:3; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BACTERIOPHAGES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EVOLUTION
BODY
CLONING
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
DNA SEQUENCING
DNA-CLONING
ELECTROPHORESIS
ENZYMES
GENETIC MAPPING
GLANDS
HEMIACETAL DEHYDROGENASES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIVER
MAMMALS
MAN
MAPPING
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
RECOMBINANT DNA
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
VIRUSES