A radioactive method for the measurement of trypsin and trypsin-like activities
Journal Article
·
· Analytical Biochemistry; (USA)
- Universidad de Buenos Aires (Argentina)
A simple and highly sensitive method for the assay of trypsin has been developed by making use of the phosphorylated synthetic peptide Leu-Arg-Arg-Ala-Ser-({sup 32}P)-Leu-Gly as substrate. The technique has been adapted from the phosphocellulose method of R. Roskoski, Jr. used for measuring of protein kinases. In addition to measuring the activity of trypsin at the microgram level, the {sup 32}P-labeled peptide method can be used for measuring other trypsin-like enzymes. It has been successfully utilized for the identification of a new peptidase from the fungus Saccobolus platensis.
- OSTI ID:
- 5673423
- Journal Information:
- Analytical Biochemistry; (USA), Journal Name: Analytical Biochemistry; (USA) Vol. 179:1; ISSN ANBCA; ISSN 0003-2697
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MOLECULAR STRUCTURE
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
RADIOASSAY
RADIOISOTOPES
SERINE PROTEINASES
TRACER TECHNIQUES
TRYPSIN
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MOLECULAR STRUCTURE
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
RADIOASSAY
RADIOISOTOPES
SERINE PROTEINASES
TRACER TECHNIQUES
TRYPSIN