Sequences of tryptic peptides containing the five cysteinyl residues of ribulosebisphosphate carboxylase/oxygenase from Rhodospirillum rubrum

Journal Article · Thu Jan 01 00:00:00 EST 1981 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(81)90536-1· OSTI ID:5301281

Stringer, C D; Norton, I L; Hartman, F C

Tryptic peptides which account for all five cysteinyl residues in ribulosebisphosphate carboxylase/oxygenase from Rhodospirillum rubrum have been purified and sequenced. Collectively, these peptides contain 94 of the approximately 500 amino acid residues per molecule of subunit. Due to one incomplete cleavage at a site for trypsin and two incomplete chymotryptic-like cleavages, eight major radioactive peptides (rather than five as predicted) were recovered from tryptic digests of the enzyme that had been carboxymethylated with (/sup 3/H)iodoacetate. The established sequences are: Gly-Tyr-Thr-Ala-Phe/Val-His-Cys*-Lys, Try-Val-Asp-Leu-Ala-Leu-Lys/Glu-Glu-Asp-Leu-Ile-Ala-Gly-Gly-Glu-His-Val-Leu-Cys*-Ala-Try, Ala-Gly-Try-Gly-Tyr/Val-Ala-Thr-Ala-Ala-His-Phe-Ala-Ala-Glu-Ser-Ser-Thr-Gly-Thr-Asp-Val-Glu-Val-Cys*-Thr-Thr-Asx-Asx-Phe-Thr-Arg, Ala-Cys*-Thr-Pro-Ile-Ile-Ser-Gly-Gly-Met-Asn-Ala-Leu-Arg, Pro-Phe-Ala-Glu-Ala-Cys*-His-Ala-Phe-Trp-Leu-Gly-Gly-Asn-Phe-Ile-Lys. In these peptides, radioactive carboxymethylcysteinyl residues are denoted with asterisks and the sites of incomplete cleavage with slashes. None of the peptides appear homologous with either of two cysteinyl-containing, active-site peptides previously isolated from spinach ribulosebisphosphate carboxylase/oxygenase.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5301281
• Journal Information: Arch. Biochem. Biophys.; (United States), Vol. 208:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PEPTIDES
STRUCTURAL CHEMICAL ANALYSIS
RHODOSPIRILLUM
CARBOXYLASE
OXYGENASES
AMINO ACIDS
CLEAVAGE
CYSTEINE
LABELLED COMPOUNDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TRYPSIN
BACTERIA
CARBOXYLIC ACIDS
CRYSTAL STRUCTURE
ENZYMES
HYDROLASES
ISOTOPE APPLICATIONS
LIGASES
MICROORGANISMS
MICROSTRUCTURE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDOREDUCTASES
PEPTIDE HYDROLASES
PROTEINS
SERINE PROTEINASES
THIOLS
550201* - Biochemistry- Tracer Techniques
550701 - Microbiology- Tracer Techniques