Sequences of tryptic peptides containing the five cysteinyl residues of ribulosebisphosphate carboxylase/oxygenase from Rhodospirillum rubrum

Journal Article · Wed Dec 31 23:00:00 EST 1980 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(81)90536-1· OSTI ID:5301281

Stringer, C D; Norton, I L; Hartman, F C

Tryptic peptides which account for all five cysteinyl residues in ribulosebisphosphate carboxylase/oxygenase from Rhodospirillum rubrum have been purified and sequenced. Collectively, these peptides contain 94 of the approximately 500 amino acid residues per molecule of subunit. Due to one incomplete cleavage at a site for trypsin and two incomplete chymotryptic-like cleavages, eight major radioactive peptides (rather than five as predicted) were recovered from tryptic digests of the enzyme that had been carboxymethylated with (/sup 3/H)iodoacetate. The established sequences are: Gly-Tyr-Thr-Ala-Phe/Val-His-Cys*-Lys, Try-Val-Asp-Leu-Ala-Leu-Lys/Glu-Glu-Asp-Leu-Ile-Ala-Gly-Gly-Glu-His-Val-Leu-Cys*-Ala-Try, Ala-Gly-Try-Gly-Tyr/Val-Ala-Thr-Ala-Ala-His-Phe-Ala-Ala-Glu-Ser-Ser-Thr-Gly-Thr-Asp-Val-Glu-Val-Cys*-Thr-Thr-Asx-Asx-Phe-Thr-Arg, Ala-Cys*-Thr-Pro-Ile-Ile-Ser-Gly-Gly-Met-Asn-Ala-Leu-Arg, Pro-Phe-Ala-Glu-Ala-Cys*-His-Ala-Phe-Trp-Leu-Gly-Gly-Asn-Phe-Ile-Lys. In these peptides, radioactive carboxymethylcysteinyl residues are denoted with asterisks and the sites of incomplete cleavage with slashes. None of the peptides appear homologous with either of two cysteinyl-containing, active-site peptides previously isolated from spinach ribulosebisphosphate carboxylase/oxygenase.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5301281
• Journal Information: Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 208:2; ISSN ABBIA
• Country of Publication: United States
• Language: English

Related Subjects

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550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BACTERIA
CARBOXYLASE
CARBOXYLIC ACIDS
CLEAVAGE
CRYSTAL STRUCTURE
CYSTEINE
ENZYMES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIGASES
MICROORGANISMS
MICROSTRUCTURE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PEPTIDE HYDROLASES
PEPTIDES
PROTEINS
RHODOSPIRILLUM
SERINE PROTEINASES
STRUCTURAL CHEMICAL ANALYSIS
THIOLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TRYPSIN