Primary structure of the human pancreatic secretory trypsin inhibitor

Journal Article · Sat Jan 01 04:00:00 EST 1977 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(77)90103-5· OSTI ID:7321095

Bartelt, D C; Shapanka, R; Greene, L J

The amino acid sequence of human pancreatic secretory trypsin inhibitor (Pubols, M. H., Bartelt, D. C., and Greene, L. J. (1974) J. Biol. Chem., 249, 2235-2242) was determined by a combination of selective trypsin and chymotrypsin hydrolysis reactions on the S-2-aminoethylcysteinyl inhibitor and conventional methods (subtractive Edman degradation and expopeptidase hydrolysis) for sequence determination of small peptides. The peptides were ordered on the basis of the identification of the amino- and carboxy-terminal residues of the products at each stage of the degradation procedure. The sequence determination was carried out on a mixture of chromatographic forms present in both tissue and pancreatic juice which are identical in amino acid composition, amino-terminal residues, molecular weight, and specific activity, but differ only in asparagine content and susceptibility to enzymatic hydrolysis. The amino acid sequence of the human inhibitor corresponding to chromatographic form A/sub 3/ has been shown to the NH/sub 2/-Asp-Ser-Leu-Gly-Arg-Glu-Ala-Lys-Cys-Tyr-Asn-Glu-Leu-Asn-Gly-Cys-Thr-Lys-Ile-Tyr-Asn-Pro-Val-Cys-Gly-Thr-Asp-Gly-Asp-Thr-Tyr-Pro-Asn-Glu-Cys-Val-Leu-Cys-Phe-Glu-Asn-Arg-Lys-Arg-Gln-Thr-Ser-Ile-Leu-Ile-Gln-Lys-Ser-Gly-Pro-Cys-COOH. The structure is compared with homologous inhibitors from porcine, bovine, and ovine pancreas.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 7321095
• Journal Information: Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 179; ISSN ABBIA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BODY
CHEMICAL REACTIONS
CHROMATOGRAPHY
DECOMPOSITION
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
ENZYME INHIBITORS
ENZYMES
GLANDS
HYDROLASES
HYDROLYSIS
LYSIS
MAMMALS
MAN
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDE HYDROLASES
PEPTIDES
PRIMATES
PROTEINS
SEPARATION PROCESSES
SOLVOLYSIS
TRYPSIN
VERTEBRATES