A combination of radioanalytical and biochemical methods in the investigation of the selenium metabolism

Conference · Fri Jan 01 04:00:00 EST 1988 · Transactions of the American Nuclear Society; (USA)

OSTI ID:5672359

Behne, D

As selenium is an essential element, the investigation of its metabolism is of great interest. Analytical methods have been used to determine the selenium levels in tissues of various animal species. Uptake, retention, and excretion of the element have been studied by means of tracer experiments. Considerably more detailed information on the metabolic fate of selenium can be obtained, however, if these methods are applied simultaneously and combined with biochemical procedures for the investigation of biologically important selenium compounds. In this way, the selenium metabolism in the tissues of the rat was studied. The quantitative determination of the element was carried out by instrumental neutron activation analysis via the gamma rays of either the long-lived radioisotope {sup 75}Se (0.1-ng detection limit after a 10-day irradiation at a thermal neutron flux density of 5 {times} 10{sup 13} cm{sup {minus}2}{center dot}s{sup {minus}1}) or the short lived {sup 77m}Se (30-ng detection limit at a flux density of 1 {times} 10{sup 13} cm{sup {minus}2}{center dot}s{sup {minus}1}). For the tracer experiments, {sup 75}Se was used. In addition to these methods, procedures for the determination of the seleno-enzyme glutathione peroxidase and for the investigation of other selenoproteins were applied.

• OSTI ID: 5672359
• Report Number(s): CONF-880601--
• Conference Information: Journal Name: Transactions of the American Nuclear Society; (USA) Journal Volume: 56
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400700 -- Radiochemistry & Nuclear Chemistry
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACTIVATION ANALYSIS
ANIMALS
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BODY
CHEMICAL ANALYSIS
CHEMISTRY
CLEARANCE
ELEMENTS
EXCRETION
ISOTOPE APPLICATIONS
KIDNEYS
MAMMALS
MATERIALS
METABOLISM
ORGANS
RATS
RESEARCH PROGRAMS
RETENTION
RODENTS
SELENIUM
SEMIMETALS
TRACER TECHNIQUES
UPTAKE
VERTEBRATES