Metabolism of sup 75 Se from ( sup 75 Se) selenate into selenoproteins in rats

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5372194

Evenson, J K; Thompson, K M; Weiss, S L; Sunde, R A ^[1]

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1. Univ. of Missouri, Columbia (United States)

Since the metabolism and nutritional biochemistry of selenate has not been studied extensively, the authors used our SDS/PAGE procedure to study ({sup 75}Se) selenate distribution, retention and incorporation into selenoproteins. The authors hypothesis was that the distribution of ({sup 75}Se) selenate among tissues would differ compared to selenite, but that {sup 75}Se incorporation into selenoproteins would follow the same pattern as selenite. To test this hypothesis, male weanling rats were fed a torula yeast-based diet supplemented with 0, 0.2, or 2 {mu}g Se/g diet for 7 wk. Rats were injected iv with 50 {mu}Ci ({sup 75}Se) selenate, and killed 1, 3, 24, and 72 h later. Total {sup 75}Se retention decreased with increasing dietary Se, and was less than previously observed for ({sup 75}Se) selenite at each level of Se. Tissue {sup 75}Se recoveries for ({sup 75}Se) selenite were 22-88% of the values previously measured for ({sup 75}Se) selenite. {sup 75}Se incorporation profiles for selenate were virtually identical to those observed for selenite in all tissues, except in Se-deficient kidney cytosol where the 60 kDa {sup 75}Se selenoprotein was not the major selenoprotein. This study shows that the chemical form of selenium can affect distribution and retention, but that the form of selenium has little effect on incorporation into selenoproteins.

• OSTI ID: 5372194
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BIOSYNTHESIS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ELEMENTS
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
METABOLISM
METALLOPROTEINS
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SELENIUM
SELENIUM 75
SELENIUM ISOTOPES
SEMIMETALS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES