Identification and comparisons of selenoproteins in rat brain and mouse neuroblastoma cells

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5372731

Zinn, K; Chaudhuri, T; Trokey, D; Sunde, R; O'Dell, B ^[1]

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1. Univ. of Missouri, Columbia (United States)

Male weanling rats, fed a Se-deficient torula-yeast diet or Se-adequate diet for 5 weeks, were injected with 400 {mu}Ci of {sup 75}Se as selenite, and sacrificed 2 and 4 wk after injection. Selenoproteins identified by SDS-PAGE included 6 major {sup 75}Se-peaks in cortex and cerebellum. While Se deficiency increased {sup 75}Se labeling of brain proteins, the percentages in each of the 6 peaks were unaffected by Se status. The total Se content of deficient cortex was only 5-10% less than adequate cortex, while deficient liver Se was 25-fold lower than adequate liver. The same 6 {sup 75}Se-peaks were identified in liver, with the 23-kDa peak greatly increased in Se adequate liver. When the 23-kDa peak was excluded, the relative distribution of {sup 75}Se in the remaining peaks was similar for liver, cortex, and cerebellum. Mouse neuroblastoma cells grown with {sup 75}Se-selenite showed labeling of 6 proteins of similar molecular weight as rat cortex, but {sup 75}Se distribution was different. Overall, the liver 23-kDa protein appeared to be the only {sup 75}Se-peak greatly changed as a result of Se status.

• OSTI ID: 5372731
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ELEMENTS
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
METABOLISM
METALLOPROTEINS
MICE
NERVOUS SYSTEM
NUCLEI
NUTRITIONAL DEFICIENCY
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RATS
RODENTS
SELENIUM
SELENIUM 75
SELENIUM ISOTOPES
SEMIMETALS
SYNTHESIS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES