Arachidonate metabolism in bovine gallbladder muscle
Journal Article
·
· Prostaglandins; (United States)
Incubation of (1-/sup 14/C)arachidonic acid (AA) with homogenates of bovine gallbladder muscle generated a large amount of radioactive material having the chromatographic mobility of 6-keto-PGF1 alpha (stable product of PGI2) and smaller amounts of products that comigrated with PGF2 alpha PGE2. Formation of these products was inhibited by the cyclooxygenase inhibitor indomethacin. The major radioactive product identified by thin-layer chromatographic mobility and by gas chromatography - mass spectrometric analysis was found to be 6-keto-PGF1 alpha. The quantitative metabolic pattern of (1-/sup 14/C)PGH2 was virtually identical to that of (1-/sup 14/C)AA. Incubation of arachidonic acid with slices of bovine gallbladder muscle released labile anti-aggregatory material in the medium, which was inhibited by aspirin or 15-hydroperoxy-AA. These results indicate that bovine gallbladder muscle has a considerable enzymatic capacity to produce PGI2 from arachidonic acid.
- Research Organization:
- Department of Medical Biochemistry, Kurume University School of Medicine, Asahi-machi, Japan
- OSTI ID:
- 5672352
- Journal Information:
- Prostaglandins; (United States), Journal Name: Prostaglandins; (United States) Vol. 25:4; ISSN PRGLB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ACETYLSALICYLIC ACID
ANALGESICS
ANIMALS
ANTIPYRETICS
ARACHIDONIC ACID
BILIARY TRACT
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CATTLE
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHROMATOGRAPHY
COWS
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
DRUGS
ENZYME INHIBITORS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MASS SPECTROSCOPY
METABOLISM
MONOCARBOXYLIC ACIDS
MUSCLES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROSTAGLANDINS
RUMINANTS
SEPARATION PROCESSES
SPECTROSCOPY
SYNTHESIS
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
VERTEBRATES
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ACETYLSALICYLIC ACID
ANALGESICS
ANIMALS
ANTIPYRETICS
ARACHIDONIC ACID
BILIARY TRACT
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CATTLE
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHROMATOGRAPHY
COWS
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
DRUGS
ENZYME INHIBITORS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MASS SPECTROSCOPY
METABOLISM
MONOCARBOXYLIC ACIDS
MUSCLES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROSTAGLANDINS
RUMINANTS
SEPARATION PROCESSES
SPECTROSCOPY
SYNTHESIS
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
VERTEBRATES