Characterization of new mutants in the early part of the yeast secretory pathway isolated by a (/sup 3/H)mannose suicide selection
Journal Article
·
· J. Cell Biol.; (United States)
We have adapted a (/sup 3/H)mannose suicide selection to identify mutations in additional genes which function in the early part of the yeast secretory pathway. Thus far this protocol has led to the identification of two new genes which are implicated in this process, as well as additional alleles of previously identified genes. The new mutants, bet1 and bet2, are temperature sensitive for growth and protein transport. Thin section analysis has revealed the accumulation of a network of endoplasmic reticulum (ER) at the restrictive temperature (37/sup 0/C). Precursors of exported proteins that accumulate in the cell at 37/sup 0/C are terminally core glycosylated. These observations suggest that the transport of precursors is blocked subsequent to translocation into the ER but before entry into the Golgi apparatus. The bet1 and bet2 mutants define two new complementation groups which have the same properties as previously identified ER-accumulating mutants. This and previous findings suggest that protein exit from the ER and entry into the Golgi apparatus is a complex process requiring at least 11 genes.
- Research Organization:
- Yale Univ. School of Medicine, New Haven, CT
- OSTI ID:
- 5669997
- Journal Information:
- J. Cell Biol.; (United States), Journal Name: J. Cell Biol.; (United States) Vol. 105:4; ISSN JCLBA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID PHOSPHATASE
ALDEHYDES
BIOLOGICAL PATHWAYS
CARBOHYDRATES
CELL CONSTITUENTS
CHEMICAL COMPOSITION
ENDOPLASMIC RETICULUM
ENZYMES
ESTERASES
FUNGI
GENE MUTATIONS
GENES
HEXOSES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MANNOSE
MEMBRANE TRANSPORT
MICROORGANISMS
MOLECULAR WEIGHT
MONOSACCHARIDES
MUTANTS
MUTATIONS
ORGANIC COMPOUNDS
ORGANOIDS
PEPTIDES
PHOSPHATASES
PLANTS
PROTEINS
SACCHARIDES
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SECRETION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
YEASTS
59 BASIC BIOLOGICAL SCIENCES
ACID PHOSPHATASE
ALDEHYDES
BIOLOGICAL PATHWAYS
CARBOHYDRATES
CELL CONSTITUENTS
CHEMICAL COMPOSITION
ENDOPLASMIC RETICULUM
ENZYMES
ESTERASES
FUNGI
GENE MUTATIONS
GENES
HEXOSES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MANNOSE
MEMBRANE TRANSPORT
MICROORGANISMS
MOLECULAR WEIGHT
MONOSACCHARIDES
MUTANTS
MUTATIONS
ORGANIC COMPOUNDS
ORGANOIDS
PEPTIDES
PHOSPHATASES
PLANTS
PROTEINS
SACCHARIDES
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SECRETION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
YEASTS