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Secretory process in Brunner's glands during recovery from stimulation with a single dose of pilocarpine

Journal Article · · Anat. Rec.; (United States)
;
The secretory pathway and kinetics of the secretory process were studied in Brunner's glands of mice after stimulation of secretion with a parasympathomimetic drug. Adult male mice were injected with pilocarpine. The animals were subsequently administered an intravenous injection of /sup 3/H-threonine, and tissue was prepared for electron microscope autoradiography at intervals ranging from 5 minutes to 2 hours after injection of the radioactive precursor. Stimulation with pilocarpine resulted in discharge of secretory granules, which was reflected in a significantly lower percentage of the cell volume occupied by granules. In both control and stimulated mice, at 5 minutes after injection of /sup 3/H-threonine, the highest percentage of silver grains was found over the rough endoplasmic reticulum. The proportion of silver grains over the rough endoplasmic reticulum declined at later intervals, and a peak of labeling was observed over the Golgi apparatus at 1 hour. Labeling of the secretory granules increased in the 1- and 2-hour samples from both control and stimulated mice, although the relative concentration of radioactivity in both Golgi-associated and apical secretory granules was greater in stimulated than control glands at 1 hour. The results suggest that the secretory protein produced by Brunner's glands was synthesized by the rough endoplasmic reticulum and transported to the Golgi apparatus where secretory granules were formed in both stimulated and control glands. Depletion of secretory granules by prior stimulation resulted in no change in the kinetics of arrival of radioactivity in the cell organelles involved in the secretory process. However, the drainage of the radioactive label from the rough endoplasmic reticulum was significantly slower in the stimulated glands than in the controls.
Research Organization:
Department of Anatomy, School of Medicine, University of Virginia, Charlottesville
OSTI ID:
5505024
Journal Information:
Anat. Rec.; (United States), Journal Name: Anat. Rec.; (United States) Vol. 206:3; ISSN ANREA
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550301* -- Cytology-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALOIDS
AMINO ACIDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DIGESTIVE SYSTEM
DRUGS
ELECTRON MICROSCOPY
ENDOPLASMIC RETICULUM
GASTROINTESTINAL TRACT
GLANDS
HYDROXY ACIDS
INTESTINES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MICE
MICROSCOPY
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PARASYMPATHOMIMETICS
PILOCARPINE
RODENTS
SECRETION
SMALL INTESTINE
THREONINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES