New mutants of Saccharomyces cerevisiae affected in the transport of proteins from the endoplasmic reticulum to the Golgi complex

Journal Article · Thu Feb 01 04:00:00 EST 1996 · Genetics

OSTI ID:543040

Wuestehube, L J; Duden, R; Eun, A ^[1]

---

1. Univ. of California, Berkeley, CA (United States); and others

We have isolated new temperature-sensitive mutations in five complementation groups, sec31-sec35, that are defective in the transport of proteins from the endoplasmic reticulum (ER) to the Golgi complex. The sec31-sec35 mutants and additional alleles of previously identified sec and vacuolar protein sorting (vps) genes were isolated in a screen based on the detection of {alpha}-factor precursor in yeast colonies replicated to and lysed on nitrocellulose filters. Secretory protein precursors accumulated in sec31-sec35 mutants at the nonpermissive temperature were core-glycosylated but lacked outer chain carbohydrate, indicating that transport was blocked after translocation into the ER but before arrival in the Golgi complex. Electron microscopy revealed that the newly identified sec mutants accumulated vesicles and membrane structures reminiscent of secretory pathway organelles. Complementation analysis revealed that sec32-1 is an allele of BOS1, a gene implicated in vesicle targeting to the Golgi complex, and sec33-1 is an allele of RET1, a gene that encodes the {alpha} subunit of coatomer. 60 refs., 8 figs., 3 tabs.

• OSTI ID: 543040
• Journal Information: Genetics, Journal Name: Genetics Journal Issue: 2 Vol. 142; ISSN GENTAE; ISSN 0016-6731
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
ELECTRON MICROSCOPY
ENDOPLASMIC RETICULUM
GENE MUTATIONS
GENES
GOLGI COMPLEXES
GROWTH
MEMBRANE TRANSPORT
MORPHOLOGY
MUTANTS
PHENOTYPE
PRECURSOR
PROTEINS
SACCHAROMYCES CEREVISIAE
STRAINS
STRUCTURE-ACTIVITY RELATIONSHIPS
TEMPERATURE DEPENDENCE