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Influence of secretagogues on asynchronous secretion of newly synthesized pancreatic proteins in the conscious rat

Journal Article · · Pancreas; (United States)
;

The secretion of newly synthesized pancreatic enzymes was studied in pancreatic duct cannulated rats after intravenous injection of 100 microCi of (/sup 35/S)methionine. Secretion rate was stimulated by intravenous infusion of either cerulein (0.2 microgram/kg h) or carbachol (10 nmol/kg h) starting simultaneously with or 180 min before the injection of the labeled methionine. Secretory proteins were analyzed by sodium dodecyl sulfate (SDS) gel electrophoresis or by nondenaturing gel electrophoresis followed by determination of the radioactivity associated with the individual proteins. Similar to unstimulated controls in all experiments, an early secretion of newly synthesized trypsinogen and chymotrypsinogen was found, whereas amylase and lipase were secreted only after a certain lag period. The results suggest that the intracellular transit of endoproteases is faster than that of other enzymes, irrespective of whether or not secretagogues were applied.

Research Organization:
Univ. of Marburg, Germany, F.R.
OSTI ID:
5669854
Journal Information:
Pancreas; (United States), Journal Name: Pancreas; (United States) Vol. 2:5; ISSN PANCE
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AMYLASE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DRUGS
ELECTROPHORESIS
ENDOCRINE GLANDS
ENZYMES
ESTERASES
EVEN-ODD NUCLEI
GLANDS
GLYCOSYL HYDROLASES
HYDROLASES
INJECTION
INTAKE
INTRAVENOUS INJECTION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPASE
LIPOTROPIC FACTORS
MAMMALS
METHIONINE
NUCLEI
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PANCREAS
PEPTIDE HYDROLASES
RADIOISOTOPES
RATS
RODENTS
SECRETION
SERINE PROTEINASES
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
TRYPSIN
VERTEBRATES