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Effects of 5-fluorouracil on the secretory process of the rat parotid gland

Thesis/Dissertation ·
OSTI ID:6792187
Experimental animals were injected intraperitoneally with 100 mg/kg 5-fluorouracil for three days. The total volume, amylase and protein content of cannulated parotid saliva were determined following stimulation with either 5 mg/kg pilocarpine or 5 mg/kg isoproterenol in experimental, pair-fed , and control animals. Saliva from experimental animals was significantly lower in volume, amylase and protein content than both control groups. 5-fluorouracil treatment reduced the total glandular amylase per unit DNA in both unstimulated and isoproterenol-stimulated parotid glands. Decreased protein synthesis may be the mechanism underlying depleted secretory protein stores since the contents of isolated secretory granules from experimental parotid glands contained less radiolabelled protein than either control group and whole gland homogenates showed marked reductions in the activities of three lysosomal enzymes and total RNA content. Experimental animals contained less labelled protein in their secretory granules than controls, but secreted a greater proportion of their total glandular radiolabelled secretory protein into saliva relative to amylase suggesting that newly synthesized secretory proteins are preferentially secreted.
Research Organization:
Illinois Univ., Urbana (USA)
OSTI ID:
6792187
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMYLASE
ANIMALS
ANTIMETABOLITES
AZINES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BODY
BODY FLUIDS
CHEMISTRY
DRUGS
ELECTROPHORESIS
ENZYMES
FLUOROURACILS
GLANDS
GLYCOSYL HYDROLASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
RATS
RODENTS
SALIVA
SALIVARY GLANDS
SECRETION
SYNTHESIS
URACILS
VERTEBRATES