The role of secretory granules in radiation-induced dysfunction of rat salivary glands

Journal Article · Wed Feb 01 04:00:00 EST 1995 · Radiation Research

DOI:https://doi.org/10.2307/3579045· OSTI ID:68620

Peter, B; Van Waarde, M A.W.H.; Konings, A W.T. ^[1]; Vissink, A ^[1]; `s-Gravenmade, E J ^[2]

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1. Univ. of Groningen (Netherlands)
2. Univ. Hospital, Groningen (Netherlands)

To investigate the possible role of secretory granules in radiation-induced salivary gland dysfunction, rats were pretreated with isoproterenol (5 mg/kg intraperitoneally) to degranulate salivary gland acini. At maximal depletion, salivary glands were locally irradiated with a single dose of 15 Gy of X rays. Parotid and submandibular/sublingual saliva samples were collected before and 1-10 days after irradiation. The lag phase, flow rate, concentrations of potassium and sodium, and amylase secretion were determined. Sham-treated, isoproterenol-treated and irradiated animals provided reference data. In the parotid gland, but not in the submandibular gland, protection against radiation-induced changes in flow rate and composition of saliva occurred after pretreatment with isoproterenol. Combining morphological data from a previous study with data from the current study, it is suggested that improvement of parotid gland function is attributed predominantly to a proliferative stimulus on acinar cells by isoproterenol and not to its degranulation effect. After pretreatment with isoproterenol, an earlier expression of radiation-induced acinar cell damage leading to death was observed, followed by a faster tissue recovery. Thus the proliferative stimulus on acinar cells may accelerate the unmasking of latent lethal damage, resulting in the earlier replacement of dead cells by new, functionally intact cells. 33 refs., 2 figs.

• Sponsoring Organization: USDOE
• OSTI ID: 68620
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 2 Vol. 141; ISSN 0033-7587; ISSN RAREAE
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
AMYLASE
FLOW RATE
MORPHOLOGICAL CHANGES
QUANTITATIVE CHEMICAL ANALYSIS
RADIATION INJURIES
RADIOPROTECTIVE SUBSTANCES
RATS
RESPONSE MODIFYING FACTORS
SALIVA
SALIVARY GLANDS
SECRETION
X RADIATION