Amylase and chymotrypsinogen synthesis and secretion by the anesthetized rat pancreas

Journal Article · Wed Dec 31 23:00:00 EST 1986 · Pancreas; (United States)

DOI:https://doi.org/10.1097/00006676-198709000-00014· OSTI ID:5669837

Iovanna, J; Giorgi, D; Dagorn, J C

To investigate the origin of nonparallel secretion, pancreatic juice was collected in the anesthetized rat, during infusion of (/sup 3/H)phenylalanine. In the basal state, the amylase to chymotrypsinogen ratios of activities and of (/sup 3/H) incorporations were 3.5 and 2.5 times higher than in the homogenate, respectively. Both ratios decreased to the value in the homogenate upon caerulein stimulation (600 ng/kg). Inhibition of protein synthesis by cycloheximide (15 ng/kg) did not alter enzyme secretion ratios, and depressed basal protein output only partly, suggesting similar secretory pathways for basal and stimulated secretion. Finally, when (/sup 3/H)phenylalanine was given before anesthesia, the amylase to chymotrypsinogen ratio of incorporations was again higher in basal secretion than in homogenate, even when further protein synthesis was blocked by cycloheximide before urethane injection. Hence, basal secretion comes from a pancreatic compartment which is functional, although minor, in the conscious animal, and shows a higher rate of amylase synthesis, compared to chymotrypsinogen, than the rest of the gland. It could consist of a subpopulation of acinar cells.

• Research Organization: Unite de Recherches de Pathologie Digestive U 31 INSERM, Marseille, France
• OSTI ID: 5669837
• Journal Information: Pancreas; (United States), Journal Name: Pancreas; (United States) Vol. 2:5; ISSN PANCE
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AMYLASE
ANESTHESIA
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOCHEMISTRY
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CHEMISTRY
CYCLOHEXIMIDE
DIGESTIVE SYSTEM
DRUGS
ENDOCRINE GLANDS
ENZYME ACTIVITY
ENZYMES
FUNGICIDES
GLANDS
GLYCOSYL HYDROLASES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDE HYDROLASES
PESTICIDES
PHENYLALANINE
QUANTITY RATIO
RATS
RODENTS
SECRETION
SERINE PROTEINASES
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TRYPSIN
VERTEBRATES