Activation and suppression of cellular immunity during reparative liver regeneration in mice
The authors study the effect of partial hepatectomy (PHE) on the functional activity of various lymphocyte populations in vitro and in vivo, namely its effect on proliferative, effector and killer activity. A mixture of 50 microliters of reacting spleen cells and 100 microliters of stimulating spleen cells from mice, irradiated in a dose of 2000 rads, was incubated as described. /sup 3/H-thymidine was added to the medium before the end of incubation. The radioactivity of the samples was measured in a beta-spectrometer. Activity of immune and normal killer cells was determined in the membrane-toxic test with /sup 3/H-uridine. Depression of killer activity and of the graft versus host reaction after PHE is evidently due to the action of suppressor cells as is shown by the example of lowering of the helper function in mixed lymphocyte culture.
- Research Organization:
- Smolensk Medical Institute, USSR
- OSTI ID:
- 5669494
- Journal Information:
- Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Vol. 101:5; Other Information: Translated from Byulleten Ehksperimental'noj Biologii i Meditsiny; 101: No. 5, 591-594(May 1986)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LIVER CELLS
BIOLOGICAL REGENERATION
IMMUNE REACTIONS
RADIOIMMUNOASSAY
LYMPHOCYTES
MICE
EXTERNAL IRRADIATION
SPLEEN CELLS
BIOLOGICAL RADIATION EFFECTS
GRAFT-HOST REACTION
TRANSPLANTS
BETA SPECTRA
CELL CULTURES
CELL PROLIFERATION
CYTOCHEMISTRY
IMMUNOSUPPRESSION
LIVER
PENICILLIN
STREPTOMYCIN
THYMIDINE
TRITIUM COMPOUNDS
UPTAKE
URIDINE
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AZINES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHEMISTRY
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
DRUGS
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
IRRADIATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIATION EFFECTS
RADIOASSAY
RADIOIMMUNOLOGY
RECOVERY
RIBOSIDES
RODENTS
SOMATIC CELLS
SPECTRA
TRACER TECHNIQUES
URACILS
VERTEBRATES
550301* - Cytology- Tracer Techniques
560120 - Radiation Effects on Biochemicals
Cells
& Tissue Culture