Activation and suppression of cellular immunity during reparative liver regeneration in mice

Journal Article · Wed Oct 01 00:00:00 EDT 1986 · Bull. Exp. Biol. Med. (Engl. Transl.); (United States)

DOI:https://doi.org/10.1007/BF00835890· OSTI ID:5669494

Rakhmilevich, A L; Sidorenko, O N

The authors study the effect of partial hepatectomy (PHE) on the functional activity of various lymphocyte populations in vitro and in vivo, namely its effect on proliferative, effector and killer activity. A mixture of 50 microliters of reacting spleen cells and 100 microliters of stimulating spleen cells from mice, irradiated in a dose of 2000 rads, was incubated as described. /sup 3/H-thymidine was added to the medium before the end of incubation. The radioactivity of the samples was measured in a beta-spectrometer. Activity of immune and normal killer cells was determined in the membrane-toxic test with /sup 3/H-uridine. Depression of killer activity and of the graft versus host reaction after PHE is evidently due to the action of suppressor cells as is shown by the example of lowering of the helper function in mixed lymphocyte culture.

• Research Organization: Smolensk Medical Institute, USSR
• OSTI ID: 5669494
• Journal Information: Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Journal Name: Bull. Exp. Biol. Med. (Engl. Transl.); (United States) Vol. 101:5; ISSN BEXBA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
560120 -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AZINES
BETA SPECTRA
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL CULTURES
CELL PROLIFERATION
CHEMISTRY
CONNECTIVE TISSUE CELLS
CYTOCHEMISTRY
DIGESTIVE SYSTEM
DRUGS
EXTERNAL IRRADIATION
GLANDS
GRAFT-HOST REACTION
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNE REACTIONS
IMMUNOASSAY
IMMUNOLOGY
IMMUNOSUPPRESSION
IRRADIATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LIVER
LIVER CELLS
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PENICILLIN
PYRIMIDINES
RADIATION EFFECTS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RECOVERY
RIBOSIDES
RODENTS
SOMATIC CELLS
SPECTRA
SPLEEN CELLS
STREPTOMYCIN
THYMIDINE
TRACER TECHNIQUES
TRANSPLANTS
TRITIUM COMPOUNDS
UPTAKE
URACILS
URIDINE
VERTEBRATES