Human lactation: maternal transfer of dietary triglycerides labeled with stable isotopes

Journal Article · Thu Oct 01 04:00:00 EDT 1987 · J. Lipid Res.; (United States)

OSTI ID:5653585

Hachey, D L; Thomas, M R; Emken, E A; Garza, C; Brown-Booth, L; Adlof, R O; Klein, P D

A stable isotope tracer method was utilized to measure quantitatively the secretion of diet-derived fatty acids (FA) into human milk. A mixture of (/sup 2/H6)tripalmitin, (/sup 2/H18)-triolein, and (/sup 2/H12)trilinolein was administered to three healthy, lactating women 22 to 30 years of age. Milk and blood samples were collected sequentially for 72 hr. The FA composition and concentration of total plasma, lipoprotein, and milk triglycerides were determined by gas-liquid chromatography (GLC) and the isotopic enrichment was determined by gas-liquid chromatography-mass spectrometry (GLC-MS). There were no statistically significant differences in mammary secretion of the individual fats, either by a single individual or between subjects. The mean secretion of fat by one breast was 5.11 +/- 1.26% of the dose (CV = 25%). There was a significant 6.0-hr delay between peak occurrence of the tracer in plasma and its occurrence in milk. The lipids are transported to the mammary gland primarily by the chylomicron and very low density lipoprotein triglycerides.

• Research Organization: Baylor College of Medicine, Houston, TX
• OSTI ID: 5653585
• Journal Information: J. Lipid Res.; (United States), Journal Name: J. Lipid Res.; (United States) Vol. 28:10; ISSN JLPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL MATERIALS
BODY FLUIDS
CHEMICAL COMPOSITION
CHROMATOGRAPHY
DEUTERIUM
ESTERS
FEMALES
FOOD
GAS CHROMATOGRAPHY
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LACTATION
LIGHT NUCLEI
LIPIDS
MAMMALS
MAN
MASS SPECTROSCOPY
MATERIALS
MEMBRANE TRANSPORT
MILK
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PREGNANCY
PRIMATES
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
TRACER TECHNIQUES
TRIGLYCERIDES
VERTEBRATES
WOMEN