Calmodulin stimulation of calcium transport in carrot microsomal vesicles. [Daucus carota]
Conference
·
· Plant Physiol., Suppl.; (United States)
OSTI ID:5653383
ATP-dependent /sup 45/Ca/sup 2 +/ uptake into microsomal vesicles isolated from cultured carrot cells (Daucus carota Danvers) was stimulated 2-3 fold by 5 ug/ml calmodulin (CaM). Microsomal vesicles separated with a linear sucrose gradient showed two peaks with CaM-stimulated Ca/sup 2 +/ uptake activities. One peak (at 1.12 g/cc) comigrated with the activity of the antimycin A-insensitive NADH-dependent cytochrome c reductase. This transport activity was enhanced 10-20 fold by 10 mM oxalate and appeared to be associates with vesicles derived primarily from the ER. The other peak of CaM-stimulated Ca/sup 2 +/ uptake (at 1.17 g/cc) was not affected by oxalate. These vesicles are probably derived from the plasma membrane. Preliminary experiments with the low-density vesicles (ER) vesicles, indicate that inositol-1,4,5-trisphosphate caused a transient reduction in intravesicular Ca/sup 2 +/. These results are consistent with the ER being an important site of intracellular Ca/sup 2 +/ regulation.
- Research Organization:
- Univ. of Maryland, College Park
- OSTI ID:
- 5653383
- Report Number(s):
- CONF-8707108-
- Conference Information:
- Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 83:4
- Country of Publication:
- United States
- Language:
- English
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Conference
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·
OSTI ID:5640533
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Journal Article
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Fri Jan 31 23:00:00 EST 1986
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·
OSTI ID:5742456
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Journal Article
·
Sat Jan 31 23:00:00 EST 1987
· Plant Physiol.; (United States)
·
OSTI ID:6793033
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CALCIUM
CALCIUM 45
CALCIUM ISOTOPES
CARBOXYLIC ACID SALTS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ELEMENTS
ENDOPLASMIC RETICULUM
ENZYMES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MEMBRANE TRANSPORT
MEMBRANES
METALS
NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
OXALATES
OXIDOREDUCTASES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SEPARATION PROCESSES
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CALCIUM
CALCIUM 45
CALCIUM ISOTOPES
CARBOXYLIC ACID SALTS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ELEMENTS
ENDOPLASMIC RETICULUM
ENZYMES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MEMBRANE TRANSPORT
MEMBRANES
METALS
NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
OXALATES
OXIDOREDUCTASES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SEPARATION PROCESSES
TRACER TECHNIQUES