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Title: Calcium transport in vesicles from carrot cells: Stimulation by calmodulin and phosphatidylserine. [Daucus carota cv. Danvers]

Abstract

The transport properties of Ca-pumping ATPases from carrot (Daucus carota cv. Danvers) tissue culture cells were studied. ATP dependent Ca transport in vesicles that comigrated with an ER marker, was stimulated 3-4 fold by calmodulin. Cyclopiazonic acid (a specific inhibitor of the sarcoplasmic/endoplasmic reticulum Ca-ATPase) partially inhibited oxalate-stimulated Ca transport activity; however, it had little or not effect on calmodulin-stimulated Ca uptake. The results suggested the presence of two types of Ca ATPases, and ER- and a plasma membrane-type. Incubation of membranes with (gamma{sup 32}P)ATP resulted in the formation of a single acyl ({sup 32}P) phosphoprotein of 120 kDa. Formation of this phosphoprotein was dependent on Ca, and enhanced by La {sup 3+}, characteristic of the plasma membrane CaATPase. Acidic phospholipids, like phosphatidylserine, stimulated Ca transport, similar to their effect on the erythrocyte plasma membrane CaATPase. These results would indicate that the calmodulin-stimulated Ca transport originated in large part from a plasma membrane-type Ca pump of 120 kDa.

Authors:
;  [1]
  1. (Univ. of Maryland, College Park (United States))
Publication Date:
OSTI Identifier:
5640533
Report Number(s):
CONF-9107184-
Journal ID: ISSN 0079-2241; CODEN: PPYSA
DOE Contract Number:
FG05-86ER13461
Resource Type:
Conference
Resource Relation:
Journal Name: Plant Physiology, Supplement; (United States); Journal Volume: 96:1; Conference: Annual meeting of the American Society of Plant Physiology, Albuquerque, NM (United States), 28 Jul - 1 Aug 1991
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CALCIUM; BIOCHEMICAL REACTION KINETICS; PLANT CELLS; PHYSIOLOGY; ATP; ATP-ASE; CALMODULIN; CARROTS; PHOSPHOLIPIDS; PHOSPHORUS 32; ACID ANHYDRASES; ALKALINE EARTH METALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ELEMENTS; ENZYMES; ESTERS; FOOD; HYDROLASES; ISOTOPES; KINETICS; LIGHT NUCLEI; LIPIDS; MAGNOLIOPHYTA; MAGNOLIOPSIDA; METALS; NUCLEI; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; PHOSPHOHYDROLASES; PHOSPHORUS ISOTOPES; PLANTS; PROTEINS; RADIOISOTOPES; REACTION KINETICS; VEGETABLES; 550200* - Biochemistry

Citation Formats

Wenling Hsieh, and Sze, Heven. Calcium transport in vesicles from carrot cells: Stimulation by calmodulin and phosphatidylserine. [Daucus carota cv. Danvers]. United States: N. p., 1991. Web.
Wenling Hsieh, & Sze, Heven. Calcium transport in vesicles from carrot cells: Stimulation by calmodulin and phosphatidylserine. [Daucus carota cv. Danvers]. United States.
Wenling Hsieh, and Sze, Heven. Wed . "Calcium transport in vesicles from carrot cells: Stimulation by calmodulin and phosphatidylserine. [Daucus carota cv. Danvers]". United States. doi:.
@article{osti_5640533,
title = {Calcium transport in vesicles from carrot cells: Stimulation by calmodulin and phosphatidylserine. [Daucus carota cv. Danvers]},
author = {Wenling Hsieh and Sze, Heven},
abstractNote = {The transport properties of Ca-pumping ATPases from carrot (Daucus carota cv. Danvers) tissue culture cells were studied. ATP dependent Ca transport in vesicles that comigrated with an ER marker, was stimulated 3-4 fold by calmodulin. Cyclopiazonic acid (a specific inhibitor of the sarcoplasmic/endoplasmic reticulum Ca-ATPase) partially inhibited oxalate-stimulated Ca transport activity; however, it had little or not effect on calmodulin-stimulated Ca uptake. The results suggested the presence of two types of Ca ATPases, and ER- and a plasma membrane-type. Incubation of membranes with (gamma{sup 32}P)ATP resulted in the formation of a single acyl ({sup 32}P) phosphoprotein of 120 kDa. Formation of this phosphoprotein was dependent on Ca, and enhanced by La {sup 3+}, characteristic of the plasma membrane CaATPase. Acidic phospholipids, like phosphatidylserine, stimulated Ca transport, similar to their effect on the erythrocyte plasma membrane CaATPase. These results would indicate that the calmodulin-stimulated Ca transport originated in large part from a plasma membrane-type Ca pump of 120 kDa.},
doi = {},
journal = {Plant Physiology, Supplement; (United States)},
number = ,
volume = 96:1,
place = {United States},
year = {Wed May 01 00:00:00 EDT 1991},
month = {Wed May 01 00:00:00 EDT 1991}
}

Conference:
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