Calcium transport in vesicles from carrot cells: Stimulation by calmodulin and phosphatidylserine. [Daucus carota cv. Danvers]
Conference
·
· Plant Physiology, Supplement; (United States)
OSTI ID:5640533
- Univ. of Maryland, College Park (United States)
The transport properties of Ca-pumping ATPases from carrot (Daucus carota cv. Danvers) tissue culture cells were studied. ATP dependent Ca transport in vesicles that comigrated with an ER marker, was stimulated 3-4 fold by calmodulin. Cyclopiazonic acid (a specific inhibitor of the sarcoplasmic/endoplasmic reticulum Ca-ATPase) partially inhibited oxalate-stimulated Ca transport activity; however, it had little or not effect on calmodulin-stimulated Ca uptake. The results suggested the presence of two types of Ca ATPases, and ER- and a plasma membrane-type. Incubation of membranes with (gamma{sup 32}P)ATP resulted in the formation of a single acyl ({sup 32}P) phosphoprotein of 120 kDa. Formation of this phosphoprotein was dependent on Ca, and enhanced by La {sup 3+}, characteristic of the plasma membrane CaATPase. Acidic phospholipids, like phosphatidylserine, stimulated Ca transport, similar to their effect on the erythrocyte plasma membrane CaATPase. These results would indicate that the calmodulin-stimulated Ca transport originated in large part from a plasma membrane-type Ca pump of 120 kDa.
- DOE Contract Number:
- FG05-86ER13461
- OSTI ID:
- 5640533
- Report Number(s):
- CONF-9107184--
- Conference Information:
- Journal Name: Plant Physiology, Supplement; (United States) Journal Volume: 96:1
- Country of Publication:
- United States
- Language:
- English
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Journal Article
·
Tue Jun 01 00:00:00 EDT 1993
· Plant Physiology; (United States)
·
OSTI ID:6247744
Calmodulin stimulation of calcium transport in carrot microsomal vesicles. [Daucus carota]
Conference
·
Tue Mar 31 23:00:00 EST 1987
· Plant Physiol., Suppl.; (United States)
·
OSTI ID:5653383
Calcium transport in tonoplast and endoplasmic reticulum vesicles isolated from cultured carrot cells. [Daucus carota Danvers]
Journal Article
·
Fri Jan 31 23:00:00 EST 1986
· Plant Physiol.; (United States)
·
OSTI ID:5742456
Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ALKALINE EARTH METALS
ATP
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CALCIUM
CALMODULIN
CARROTS
DAYS LIVING RADIOISOTOPES
ELEMENTS
ENZYMES
ESTERS
FOOD
HYDROLASES
ISOTOPES
KINETICS
LIGHT NUCLEI
LIPIDS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METALS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOHYDROLASES
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHYSIOLOGY
PLANT CELLS
PLANTS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
VEGETABLES
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ALKALINE EARTH METALS
ATP
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CALCIUM
CALMODULIN
CARROTS
DAYS LIVING RADIOISOTOPES
ELEMENTS
ENZYMES
ESTERS
FOOD
HYDROLASES
ISOTOPES
KINETICS
LIGHT NUCLEI
LIPIDS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METALS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOHYDROLASES
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHYSIOLOGY
PLANT CELLS
PLANTS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
VEGETABLES