Loss of /sup 51/chromium, lactate dehydrogenase, and /sup 111/indium as indicators of endothelial cell injury

Journal Article · Sun Nov 01 04:00:00 EST 1987 · Lab. Invest.; (United States)

OSTI ID:5649397

Chopra, J; Joist, J H; Webster, R O

Injury to endothelial cells appears to be an important initial event in the pathogenesis of many diseases such as acute lung injury, venous and arterial thromboembolism, and atherosclerosis. Different methods for detecting damage to cultured endothelial cells have been described. However, their relative sensitivity as markers of endothelial cell damage has not been adequately determined. We compared the loss of /sup 51/Chromium (/sup 51/Cr), the cytoplasmic enzyme lactate dehydrogenase (LDH), and /sup 111/Indium (/sup 111/In) from endothelial cells upon exposure to several injurious agents. Cultured bovine pulmonary artery endothelial cells in confluent monolayers were labeled with /sup 51/Cr or /sup 111/Inoxine and exposed to increasing concentrations of the nonionic detergent, Triton X-100 (0.2 to 1%), hydrogen peroxide (1 to 500 microM), or neutrophils stimulated with phorbol myristate acetate. With all forms of injury, loss of /sup 51/Cr occurred earlier and to a greater extent than LDH loss which in turn was greater than loss of /sup 111/In. Substantial loss of /sup 51/Cr was observed in the absence of appreciable ultrastructural damage to endothelial cell external membranes. The findings may reflect the relative ease with which small molecules such as adenine nucleotides (/sup 51/Cr-labeled) escape whereas larger molecules such as LDH and proteins binding /sup 111/In are retained intracellularly. Thus, /sup 51/Cr loss appears to be a more sensitive indicator of sublytic endothelial cell injury than either /sup 111/In or LDH release.

• Research Organization: St. Louis University School of Medicine, MO
• OSTI ID: 5649397
• Journal Information: Lab. Invest.; (United States), Journal Name: Lab. Invest.; (United States) Vol. 57:5; ISSN LAINA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL TISSUES
AROMATICS
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL INDICATORS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARCINOGENS
CELL CULTURES
CHROMIUM 51
CHROMIUM ISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
ENZYMES
ESTERS
EVEN-ODD NUCLEI
HEMIACETAL DEHYDROGENASES
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
HYDROXY COMPOUNDS
INDIUM 111
INDIUM ISOTOPES
INJURIES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LACTATE DEHYDROGENASE
LEUKOCYTES
MATERIALS
MINUTES LIVING RADIOISOTOPES
MORPHOLOGICAL CHANGES
NEUTROPHILS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
OXINE
OXYGEN COMPOUNDS
PATHOGENESIS
PEROXIDES
PHORBOL ESTERS
PYRIDINES
QUINOLINES
RADIOISOTOPES
RESPIRATORY SYSTEM DISEASES
TISSUES
TOXICITY
TRACER TECHNIQUES
ULTRASTRUCTURAL CHANGES
VASCULAR DISEASES