sup 51 Cr loss and lactate dehydrogenase (LDH) release in irradiated human tumor cells

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5375573

Ts'ao, C; Molteni, A; Hinz, J ^[1]

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1. Northwestern Medical School, Chicago, IL (United States)

Much of what is known about tumor cell radiosensitivity in vitro derives from the colony formation assay. Other endpoints of cytotoxicity in irradiated tumor cells are rarely examined. The purpose of this study was to determine whether loss of {sup 51}Cr from prelabeled cells and release of LDH could be used to quantify radiation injury in two cultured human tumor cell lines: a prostate carcinoma and a melanoma. Bovine aortic endothelial cells (EC) known to release {sup 51}Cr and LDH following irradiation, were cotested. Radioactivity and LDH activity in the culture medium were determined after 0-40 Gy of {sup 60}CO {gamma} rays. Proliferation of irradiated tumor cells was also studied. EC exhibited a time- and radiation dose-dependent increase in {sup 51}Cr and LDH release. Both tumor cell lines showed a time-dependent increase in {sup 51}Cr release, but this baseline release was not elevated after irradiation. LDH release from the prostate cancer cell line was observed within 8 hr after 40 Gy, and at 48 hr by 10 Gy. Irradiated melanoma cells, in contrast, never release excess LDH into the culture medium. Melanoma cells continued to proliferate after 10 Gy, while proliferation of prostate cancer cells was totally arrested by this dose of exposure. While {sup 51}Cr loss and LDH release appear to be sensitive indicators of radiation-induced damage in EC, they have limited value in the assessment of radiation-induced cytotoxicity in human prostate cancer and melanoma cells.

• OSTI ID: 5375573
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AORTA
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BLOOD VESSELS
BODY
CARCINOMAS
CARDIOVASCULAR SYSTEM
CELL PROLIFERATION
CHROMIUM 51
CHROMIUM ISOTOPES
COBALT 60
COBALT ISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
ENZYMES
EVEN-ODD NUCLEI
EXPERIMENTAL NEOPLASMS
GAMMA RADIATION
GLANDS
HEMIACETAL DEHYDROGENASES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LACTATE DEHYDROGENASE
LOSSES
MALE GENITALS
MAMMALS
MAN
MELANOMAS
MINUTES LIVING RADIOISOTOPES
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PRIMATES
PROSTATE
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY
RUMINANTS
SECRETION
SHEEP
TIME DEPENDENCE
TISSUES
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES
YEARS LIVING RADIOISOT