Radioimmunoassay and chemical ionization/mass spectrometry compared for plasma cortisol determination

Journal Article · Thu Dec 31 23:00:00 EST 1981 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5639362

Lindberg, C ^[1]; Johnson, S; Hedner, P; Gustafsson, A

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1. AB Draco, Lund, Sweden

A method is described for determination of cortisol in plasma and urine, based on chemical ionization/mass spectrometry with deuterium-labeled cortisol as the internal standard. The within-run precision (CV) was 2.5-5.7%, the between-run precision 4.6%. Results by this method were compared with those by a radioimmunological method (RIANEN Cortisol, New England Nuclear) for 395 plasma samples. The latter method gave significantly higher (approx. 25%) cortisol values.

• OSTI ID: 5639362
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 28:1; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BLOOD PLASMA
BODY FLUIDS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
CORTICOSTEROIDS
DATA
DEUTERIUM COMPOUNDS
EXPERIMENTAL DATA
GAS CHROMATOGRAPHY
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
ISOTOPE APPLICATIONS
KETONES
MASS SPECTROSCOPY
MATERIALS
MEASURING METHODS
MODIFICATIONS
NUMERICAL DATA
ORGANIC COMPOUNDS
PREGNANES
RADIOASSAY
RADIOIMMUNOASSAY
SEPARATION PROCESSES
SPECTROSCOPY
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
UPTAKE
URINE
WASTES