Radioimmunoassay for digoxin with a fully automated continuous-flow system
Journal Article
·
· Clin. Chem.; (United States)
OSTI ID:5639352
We describe a fully automated continuous-flow radioimmunoassay for digoxin with use of a Technicon AutoAnalyzer system. It makes use of antibodies linked to magnetizable particles; a magnet separates the bound and free fractions. The precise dispensing of reagents by the AutoAnalyzer system permits a small incubation volume (about 160 microliter) and the reproducible timing enables non-equilibrium conditions to be used. Thus a single sample is assayed in 15 min, with a sample throughput of 30/h. The standard curve ranges from 0.5 to 8.0 microgram/liter and is most precise between 1 and 3 microgram/liter, the between-assay coefficient of variation being less than 3%. There is no significant carryover between samples of high and low concentration, and results by the method correlate closely (r = 0.969) with those by an established manual assay in which charcoal separation is used.
- Research Organization:
- St. Bartholomew's Hospital, London, England
- OSTI ID:
- 5639352
- Journal Information:
- Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 23:10; ISSN CLCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
AUTOMATION
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CARBOHYDRATES
CARDIOTONICS
CONCENTRATORS
DIGITALIS GLYCOSIDES
DIGOXIN
DRUGS
GLYCOSIDES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
MAGNETIC PROPERTIES
MAGNETIC SEPARATORS
ORGANIC COMPOUNDS
PARTICLES
PHYSICAL PROPERTIES
RADIOASSAY
RADIOIMMUNOASSAY
STEROIDS
TRACER TECHNIQUES
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
AUTOMATION
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CARBOHYDRATES
CARDIOTONICS
CONCENTRATORS
DIGITALIS GLYCOSIDES
DIGOXIN
DRUGS
GLYCOSIDES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
MAGNETIC PROPERTIES
MAGNETIC SEPARATORS
ORGANIC COMPOUNDS
PARTICLES
PHYSICAL PROPERTIES
RADIOASSAY
RADIOIMMUNOASSAY
STEROIDS
TRACER TECHNIQUES