Selection of lys2 mutants of the yeast Saccharomyces cerevisiae by the utilization of. cap alpha. -aminoadipate

Journal Article · Sat Sep 01 04:00:00 EDT 1979 · Genetics; (United States)

OSTI ID:5639162

Chattoo, B B ^[1]; Sherman, F; Azubalis, D A; Fjellstedt, T A; Mehnert, D; Ogur, M

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1. Univ. of Rochester, NY

Normal strains of Saccharomyces cerevisiae do not use ..cap alpha..-aminoadipate as a principal nitrogen source. However, ..cap alpha..-aminoadipate is utilized as a nitrogen source by lys2 and lys5 strains having complete or partial deficiencies of ..cap alpha..-aminoadipate reductase and, to a limited extent, by heterozygous lys2/ + strains. Lys2 mutants were conveniently selected on media containing ..cap alpha..-aminoadipate as a nitrogen source, lysine, and other supplements to furnish other posible auxotrophic requirements. The lys2 mutations were obtained in a variety of laboratory strains containing other markers, including other lysine mutations. In addition to the predominant class of lys2 mutants, low frequencies of lys5 mutants and mutants not having any obvious lysine requirement were recovered on ..cap alpha..-aminoadipate medium. The mutants not requiring lysine appeared to have mutations at the lys2 locus that caused partial deficiencies of ..cap alpha..-aminoadipate reductase. Such partial deficiencies are believed to be sufficiently permissive to allow lysine biosynthesis, but sufficiently restrictive to allow for the utilization of ..cap alpha..-aminoadipate. Although it is unknown why partial or complete deficiencies of ..cap alpha..-aminoadipate reductase cause utilization of ..cap alpha..-aminoadipate as a principal nitrogen source, the use of ..cap alpha..-aminoadipate medium has considerable utility as a selective medium for lys2 and lys5 mutants.

• OSTI ID: 5639162
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 93:1; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550401* -- Genetics-- Tracer Techniques
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59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AMINOADIPIC ACID
AMINOTRANSFERASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBON 14
CARBON ISOTOPES
CARBOXYLIC ACIDS
CATALYSIS
DATA
DATA FORMS
ENZYMES
EVEN-EVEN NUCLEI
EXPERIMENTAL DATA
FUNGI
GENE MUTATIONS
GROWTH
INFORMATION
ISOLATED VALUES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LYSINE
METABOLISM
MICROORGANISMS
MUTANTS
MUTATIONS
NUCLEI
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PLANTS
RADIOASSAY
RADIOISOTOPES
REACTION KINETICS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SYNTHESIS
TEMPERATURE EFFECTS
TIME DEPENDENCE
TRACER TECHNIQUES
TRANSFERASES
YEARS LIVING RADIOISOTOPES
YEASTS