Fermentation and anaerobic respiration by Rhodospirillum rubrum and Rhodopseudomonas capsulata

Journal Article · Fri Jan 01 04:00:00 EST 1982 · J. Bacteriol.; (United States)

OSTI ID:5638097

Schultz, J E; Weaver, P F

Rhodospirillum rubrum and Rhodopseudomonas capsulata were able to grow anaerobically in the dark either by a strict mixed-acid fermentation of sugars or, in the presence of an appropriate electron acceptor, by an energy-linked anaerobic respiration. Both species fermented fructose without the addition of accessory oxidants, but required the initial presence of bicarbonate before fermentative growth could begin. Major products of R. rubrum fermentation were succinate, acetate, propionate, formate, hydrogen, and carbon dioxide; R. capsulata produced major amounts of lactate, acetate, succinate, hydrogen, and carbon dioxide. R. rubrum and R. capsulata were also capable of growing strictly through anaerobic, respiratory mechanisms. Nonfermentable substrates, such as succinate, malate, or acetate, supported growth only in the presence of an electron acceptor such as dimethyl sulfoxide or trimethylamine oxide. Carbon dioxide and succinate plus dimethyl sulfoxide were produced during growth of R. rubrum and R. capsulata on succinate plus dimethyl sulfoxides. Molar growth yields from cultures grown anaerobically in the dark on fructose plus dimethyl sulfoxide were 3.8 to 4.6 times higher than values obtained from growth on fructose alone and were 56 to 6o% of thevalues obtained from aerobic, respiratory growth with fructose. Likewise, molar growth yields from anaerobic, respiratory growth conditions with succinate plus dimethyl sulfoxide were 51 to 54% of the values obtained from aerobic, respiratory growth with succinate. The data indicate that dimethyl sulfoxide or trimethylamine oxide as a terminal oxidant is approximately 33 to 41% as efficient as O/sub 2/ in conserving energy through electron transport-linked respiration.

• Research Organization: Solar Energy Research Instit., Golden, CO
• OSTI ID: 5638097
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 149:1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ACID CARBONATES
ANAEROBIC CONDITIONS
BACTERIA
BIOCONVERSION
CARBOHYDRATES
CHEMICAL REACTIONS
DATA
DMSO
EXPERIMENTAL DATA
FERMENTATION
FRUCTOSE
GROWTH
HEXOSES
INFORMATION
KETONES
METABOLISM
MICROORGANISMS
MONOSACCHARIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDIZERS
PRODUCTIVITY
REDOX REACTIONS
RESPIRATION
RESPONSE MODIFYING FACTORS
SACCHARIDES
SULFOXIDES