Nitrous oxide reduction by members of the family Rhodospirillaceae and the nitrous oxide reductase of Rhodopseudomonas capsulata

Journal Article · Fri Nov 01 04:00:00 EST 1985 · J. Bacteriol.; (United States)

OSTI ID:5448902

McEwan, A G; Greenfield, A J; Wetzstein, H G; Jackson, J B; Ferguson, S J

After growth in the absence of nitrogenous oxides under anaerobic phototrophic conditions, several strains of Rhodopseudomonas capsulata were shown to possess a nitrous oxide reductase activity. The enzyme responsible for this activity had a periplasmic location and resembled a nitrous oxide reductase purified from Pseudomonas perfectomarinus. Electron flow to nitrous oxide reductase was coupled to generation of a membrane potential and inhibited by rotenone but not antimycin. It is suggested that electron flow to nitrous oxide reductase branches at the level of ubiquinone from the previously characterized electron transfer components of R. capsulata. R. capsulata grew under dark anaerobic conditions in the presence of malate as carbon source and nitrous oxide as electron acceptor. This confirms that nitrous oxide respiration is linked to ATP synthesis. Phototrophically and anaerobically grown cultures of nondenitrifying strains of Rhodopseudomonas sphaeroides, Rhodopseudomonas palustris, and Rhodospirillum rubrum also possessed nitrous oxide reductase activity.

• Research Organization: Univ. of Birmingham, England
• OSTI ID: 5448902
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 164:2; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANAEROBIC CONDITIONS
BACTERIA
CHALCOGENIDES
CHEMICAL REACTIONS
ELECTRON TRANSFER
ENZYME ACTIVITY
ENZYMES
KINETICS
MICROORGANISMS
NITROGEN COMPOUNDS
NITROGEN OXIDES
NITROUS OXIDE
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
REDUCTION
RHODOPSEUDOMONAS
RHODOSPIRILLUM