Photochemistry and enzymology of photosynthesis

Technical Report · Mon Jul 30 04:00:00 EDT 1979

DOI:https://doi.org/10.2172/5637483· OSTI ID:5637483

Radmer, R.

In the first task, a specially designed mass spectrometer system monitors the gas exchange occurring in response to single short flashes of light. This apparatus will be primarily used to study photosystem II donor reactions, such as the photooxidation of hydroxylamine, hydrazine, and hydrogen peroxide. This technique will also be used to study the light-induced exchange of O/sub 2/ and CO/sub 2/ in algae. The second task, biochemical studies, will focus on the role of chloroplast copper in photosynthesis. We propose to isolate, purify, and characterize the chloroplast copper enzyme polyphenol oxidase, and attempt to elucidate its role in photosynthesis. These studies will be integrated with a new program devoted to the biochemical response of the photosynthetic membrane to stress. The third task is a series of studies on the light-harvesting and electron-transport mechanisms of C/sub 4/ plants. This program will address three basic problems: (1) the effect of different preparative procedures on various photosynthetic reactions, with particular emphasis on photosystem II reactions in corn bundle sheath chloroplasts; (2) the development and testing of photosystem II assays; and (3) studies of the stoichiometry of electron carriers in bundle sheath chloroplasts, and whether cyclic phosphorylation could be a major pathway in this tissue.

• Research Organization: Martin Marietta Corp., Baltimore, MD (USA); Martin Marietta Labs., Baltimore, MD (USA)
• OSTI ID: 5637483
• Report Number(s): COO-3326-82
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400500 -- Photochemistry
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CELL CONSTITUENTS
CHALCOGENIDES
CHEMICAL REACTIONS
CHEMISTRY
CHLOROPLASTS
COPPER
CRYOGENIC FLUIDS
DATA
DATA FORMS
ELECTROMAGNETIC RADIATION
ELECTRON TRANSFER
ELEMENTS
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
FLUIDS
INFORMATION
IRRADIATION
ISOLATED VALUES
KINETICS
METALS
NONMETALS
NUMERICAL DATA
OXIDES
OXIDOREDUCTASES
OXYGEN
OXYGEN COMPOUNDS
PHOSPHORYLATION
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PLANTS
PULSED IRRADIATION
RADIATIONS
REACTION KINETICS
SYNTHESIS
TRANSITION ELEMENTS
VISIBLE RADIATION