Photochemistry and enzymology of photosynthesis. Progress report and renewal proposal

Technical Report · Fri Aug 01 04:00:00 EDT 1980

OSTI ID:5030563

This work consists of three basic tasks: (1) mass spectrometer studies involving measurements in flashing light; (2) biochemical studies of chloroplast copper proteins; and (3) light harvesting and electron transport in C/sub 4/ plants. In the first task, a specially designed mass spectrometer system will be used to monitor the gas exchange occurring in response to single short flashes of light. This technique will also be used in conjunction with continuous light measurements, to study the light-induced exchange of O/sub 2/ and CO/sub 2/ in algae. The second task will continue to focus on the role of chloroplast copper in photosynthesis. A thylakoid-bound polyphenol oxidase enzyme was isolated and purified from spinach chloroplasts. The role of this enzyme in the chloroplast will be determined and some of its unusual and intriguing properties will be characterized. The third task is a series of studies related to the light-harvesting and electron-transport mechanisms of C/sub 4/ plants. Ongoing work relating to this task will be devoted to (1) the cyclic operation of photosystem I and (2) photosystem II assays, especially the oxidation of tetraphenylboron. (ERB)

• Research Organization: Martin Marietta Labs., Baltimore, MD (USA)
• DOE Contract Number: AC02-76ER03326
• OSTI ID: 5030563
• Report Number(s): DOE/ER/03326-T1; ON: DE82021992
• Country of Publication: United States
• Language: English

Related Subjects

14 SOLAR ENERGY
140505 -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400500 -- Photochemistry
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALGAE
BIOCHEMISTRY
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CELL CONSTITUENTS
CHALCOGENIDES
CHEMICAL REACTION KINETICS
CHEMICAL REACTIONS
CHEMISTRY
CHLOROPLASTS
CONFORMATIONAL CHANGES
COPPER
ELECTROMAGNETIC RADIATION
ELECTRON TRANSFER
ELEMENTS
ENZYME ACTIVITY
ENZYMES
HYDRAZINE
KINETICS
MASS SPECTROSCOPY
METALS
NITROGEN COMPOUNDS
NONMETALS
OXIDES
OXIDOREDUCTASES
OXYGEN
OXYGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOCHEMISTRY
PHOTOSYNTHESIS
PLANTS
PULSES
RADIATIONS
REACTION KINETICS
SPECTROSCOPY
SYNTHESIS
TRANSITION ELEMENTS
VISIBLE RADIATION