Hydroxyl radicals do not crosslink a DNA-lysozyme complex

Journal Article · Sun Dec 01 04:00:00 EST 1985 · Carcinogenesis; (United States)

DOI:https://doi.org/10.1093/carcin/6.12.1689· OSTI ID:5637040

Werbin, H; Cheng, C J

The ionic complex between lysozyme and either Escherichia coli DNA or pBR322 DNA was not crosslinked by two systems capable of producing nanomolar amounts of hydroxyl radicals, the oxidation of xanthine by xanthine oxidase and the iron catalyzed oxidation of ascorbic acid. Nor did effective crosslinking occur with micromolar quantities of hydroxyl radicals raised by the addition of adenosine nucleotides to ferrous iron and hydrogen peroxide. In this case, radical content was estimated by colorimetric analysis of formaldehyde following hydroxyl radical oxidation of dimethyl sulfoxide. Similar amounts of radicals generated by pulse radiolysis in a nitrous oxide atmosphere failed also to induce crosslinking. These findings do not support a role for hydroxy radicals in the N-acetoxy-2-acetylaminofluorene induced crosslinking of DNA to lysozyme proposed earlier.

• OSTI ID: 5637040
• Journal Information: Carcinogenesis; (United States), Journal Name: Carcinogenesis; (United States) Vol. 12; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
ASCORBIC ACID
AZAARENES
BACTERIA
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CHALCOGENIDES
CHEMICAL REACTIONS
CROSS-LINKING
DMSO
DNA
ENZYMES
ESCHERICHIA COLI
GLYCOSYL HYDROLASES
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROLASES
HYDROXIDES
KINETICS
LYSOZYME
MICROORGANISMS
NITROGEN COMPOUNDS
NITROGEN OXIDES
NITROUS OXIDE
NUCLEIC ACIDS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PLASMIDS
POLYMERIZATION
PURINES
RADICALS
REACTION KINETICS
SULFOXIDES
VITAMINS
XANTHINES