NADH induces the generation of superoxide radicals in leaf peroxisomes. [Pisum sativum L]

Journal Article · Tue Feb 28 23:00:00 EST 1989 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.89.3.728· OSTI ID:5027773

del Rio, L A; Sandalio, L M; Palma, J M ^[1]; Fernandez, V M; Ruperez, F L ^[2]

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1. Unidad de Bioquimica Vegetal, Granada (Spain)
2. Instituto de Catalisis, Madrid (Spain)

In peroxisomes isolated from pea leaves (Pisum sativum L.) the production of superoxide free radicals (O{sub 2}{sup {minus}}) by xanthine and NADH was investigated. In peroxisomal membranes, 100 micromolar NADH induced the production of O{sub 2}{sup {minus}} radicals. In the soluble fractions of peroxisomes, no generation of O{sub 2}{sup {minus}} radicals was observed by incubation with either NADH or xanthine, although xanthine oxidase was found located predominantly in the matrix of peroxisomes. The failure of xanthine to induce superoxide generation was probably due to the inability to fully suppress the endogenous Mn-superoxide dismutase activity by inhibitors which were inactive against xanthine oxidase. The generation of superoxide radicals in leaf peroxisomes together with the recently described production of these oxygen radicals in glyoxysomes suggests that O{sub 2}{sup {minus}} generation could be a common metabolic property of peroxisomes and further supports the existence of active oxygen-related roles for peroxisomes in cellular metabolism.

• OSTI ID: 5027773
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 89:3; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
CELL CONSTITUENTS
COENZYMES
ENZYME ACTIVITY
ENZYMES
FUNCTIONS
KINETICS
LEAVES
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METABOLISM
NADH2
NUCLEOTIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PISUM
PLANT CELLS
PLANTS
RADICALS
REACTION KINETICS
SUPEROXIDE DISMUTASE
SUPEROXIDE RADICALS