Biosynthesis of the phytoalexin pisatin. [Pisum sativum]

Journal Article · Thu Nov 01 04:00:00 EST 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.94.3.1444· OSTI ID:5628659

Preisig, C L; Bell, J N; Matthews, D E; VanEtten, H D ^[1]; Sun, Yuejin; Hrazdina, G ^[2]

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1. Cornell Univ., Ithaca, NY (USA)
2. Cornell Univ., Geneva, NY (USA)

NADPH-dependent reduction of 2{prime},7-dihydroxy-4{prime},5{prime}-methylenedioxyisoflavone to the isoflavanone sophorol, a proposed intermediate step in pisatin biosynthesis, was detected in extracts of Pisum sativum. This isoflavone reductase activity was inducible by treatment of pea seedlings with CuCl{sub 2}. The timing of induction coincided with that of the 6a-hydroxymaackiain 3-O-methyltransferase, which catalyzes the terminal biosynthetic step. Neither enzyme was light inducible. Further NADPH-dependent metabolism of sophorol by extracts of CuCl{sub 2}-treated seedlings was also observed; three products were radiolabeled when ({sup 3}H)sophorol was the substrate, one of which is tentatively identified as maackiain.

• OSTI ID: 5628659
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 94:3; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOSYNTHESIS
CHLORIDES
CHLORINE COMPOUNDS
COENZYMES
COPPER CHLORIDES
COPPER COMPOUNDS
COPPER HALIDES
ENZYME ACTIVITY
ENZYMES
HALIDES
HALOGEN COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METHYL TRANSFERASES
NADP
NUCLEOTIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PHYSIOLOGY
PISUM
PLANT GROWTH REGULATORS
PLANTS
SEEDLINGS
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
TRANSITION ELEMENT COMPOUNDS
TRITIUM COMPOUNDS