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Title: Capillaries for use in a multiplexed capillary electrophoresis system

Patent ·
OSTI ID:563680

The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification (``base calling``) is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations. 19 figs.

Research Organization:
Iowa State Univ., Ames, IA (United States)
Sponsoring Organization:
USDOE, Washington, DC (United States)
DOE Contract Number:
W-7405-ENG-82
Assignee:
Iowa State Univ. Research Foundation, Ames, IA (United States)
Patent Number(s):
US 5,695,626/A/
Application Number:
PAN: 8-667,950
OSTI ID:
563680
Resource Relation:
Other Information: PBD: 9 Dec 1997
Country of Publication:
United States
Language:
English