Multiplexed capillary electrophoresis system

Yeung, Edward S; Chang, Huan-Tsang; Fung, Eliza N; Li, Qingbo; Lu, Xiandan

Multiplexed capillary electrophoresis system

Patent · Sun Dec 31 23:00:00 EST 1995

OSTI ID:870724

Yeung, Edward S ^[1]; Chang, Huan-Tsang ^[2]; Fung, Eliza N ^[1]; Li, Qingbo ^[1]; Lu, Xiandan ^[1]

Ames, IA
Silver Spring, MD

The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification ("base calling") is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations.

View Patent

Research Organization:: Ames Laboratory (AMES), Ames, IA; Iowa State University, Ames, IA (US)

DOE Contract Number:: W-7405-ENG-82

Assignee:: Iowa State University Research Foundation, Inc. (Ames, IA)

Patent Number(s):: US 5582705

Application Number:: 08/444,565

OSTI ID:: 870724

Country of Publication:: United States

Language:: English

References (41)

A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides Prober, J. M.; Trainor, G. L.; Dam, R. J. Science, Vol. 238, Issue 4825, p. 336-341 https://doi.org/10.1126/science.2443975	journal	October 1987
High-Speed DNA Sequencer Using Capillary Gel Electrophoresis with a Laser-Induced Four-Color Fluorescent DNA Detector. Tomisaki, Riyo; Baba, Yoshinobu; Tsuhako, Mitsutomo Analytical Sciences, Vol. 10, Issue 5 https://doi.org/10.2116/analsci.10.817	journal	January 1994
Gel-filled capillaries for nucleic acid separations in capillary electrophoresis Baba, Yoshinobu; Tsuhako, Mitsutomo TrAC Trends in Analytical Chemistry, Vol. 11, Issue 8 https://doi.org/10.1016/0165-9936(92)87090-7	journal	September 1992
Capillary electrophoresis as a technique to analyze sequence-induced anomalously migrating DNA fragments Wenz, H. Michael Nucleic Acids Research, Vol. 22, Issue 19 https://doi.org/10.1093/nar/22.19.4002	journal	January 1994
Stability of capillary gels for automated sequencing of DNA Swerdlow, Harold; Dew-Jager, Kerry E.; Brady, Kevin Electrophoresis, Vol. 13, Issue 1 https://doi.org/10.1002/elps.11501301101	journal	January 1992
Separation of DNA restriction fragments by high performance capillary electrophoresis with low and zero crosslinked polyacrylamide using continuous and pulsed electric fields Heiger, David N.; Cohen, Aharon S.; Karger, Barry L. Journal of Chromatography A, Vol. 516, Issue 1 https://doi.org/10.1016/S0021-9673(01)90202-X	journal	September 1990
Separation of Fragments up to 570 Bases in Length by Use of 6% T Non-Cross-Linked Polyacrylamide for DNA Sequencing in Capillary Electrophoresis Best, Norine.; Arriaga, Edgar.; Chen, Da Yong. Analytical Chemistry, Vol. 66, Issue 22 https://doi.org/10.1021/ac00094a031	journal	November 1994
Fluorescence detection in capillary zone electrophoresis using a charge-coupled device with time-delayed integration Sweedler, J. V.; Shear, J. B.; Fishman, H. A. Analytical Chemistry, Vol. 63, Issue 5 https://doi.org/10.1021/ac00005a022	journal	March 1991
Fluorescence detection in capillary electrophoresis: evaluation of derivatizing reagents and techniques Albin, Michael.; Weinberger, Robert.; Sapp, Edwin. Analytical Chemistry, Vol. 63, Issue 5 https://doi.org/10.1021/ac00005a006	journal	March 1991
Development of novel separation techniques for biological samples in capillary electrophoresis Chang, Huan -Tsung https://doi.org/10.2172/10190677	report	July 1994
Two-label peak-height encoded DNA sequencing by capillary gel electrophoresis: three examples Chen, DaYong; Harke, Heather R.; Dovichi, Norman J. Nucleic Acids Research, Vol. 20, Issue 18 https://doi.org/10.1093/nar/20.18.4873	journal	January 1992
Compensating for instrumental and sampling biases accompanying electrokinetic injection in capillary zone electrophoresis Lee, Thomas T.; Yeung, Edward S. Analytical Chemistry, Vol. 64, Issue 11 https://doi.org/10.1021/ac00035a007	journal	June 1992
Polyhydroxy and polyethyleneglycol (meth)acrylate polymers: Physical properties and general studies for their use as electrophoresis matrices Zewert, Thomas; Harrington, Michael Electrophoresis, Vol. 13, Issue 1 https://doi.org/10.1002/elps.11501301181	journal	January 1992
Size-Selective Capillary Electrophoresis (SSCE) Separation of DNA Fragments Crehan, William A. Mac; Rasmussen, Henrik T.; Northrop, David M. Journal of Liquid Chromatography, Vol. 15, Issue 6-7 https://doi.org/10.1080/10826079208018850	journal	April 1992
Multiplex DNA sequencing Church, G.; Kieffer-Higgins, S. Science, Vol. 240, Issue 4849 https://doi.org/10.1126/science.3353714	journal	April 1988
DNA sequencing by capillary electrophoresis: use of a two-laser-two-window intensified diode array detection system Carson, S.; Cohen, A. S.; Belenkii, A. Analytical Chemistry, Vol. 65, Issue 22 https://doi.org/10.1021/ac00070a009	journal	November 1993
Expert system for data acquisition to achieve a constant signal-to-noise ratio: application to imaging of DNA sequencing gels Koutny, Lance B.; Yeung, Edward S. Analytical Chemistry, Vol. 65, Issue 2 https://doi.org/10.1021/ac00050a010	journal	January 1993
Simultaneous monitoring of DNA fragments separated by electrophoresis in a multiplexed array of 100 capillaries Ueno, Kyoji.; Yeung, Edward S. Analytical Chemistry, Vol. 66, Issue 9 https://doi.org/10.1021/ac00081a010	journal	April 1994
Native fluorescence detection of nucleic acids and DNA restriction fragments in capillary electrophoresis Milofsky, Robert E.; Yeung, Edward S. Analytical Chemistry, Vol. 65, Issue 2 https://doi.org/10.1021/ac00050a011	journal	January 1993
Capillary electrophoresis of DNA restriction fragments with solutions of entangled polymers Kleemiß, Maria H.; Gilges, Martin; Schomburg, Gerhard Electrophoresis, Vol. 14, Issue 1 https://doi.org/10.1002/elps.1150140179	journal	January 1993
Interactive control of pulsed field gel electrophoresis via real time monitoring McGregor, David A.; Yeung, Edward S. Analytical Chemistry, Vol. 64, Issue 1 https://doi.org/10.1021/ac00025a002	journal	January 1992
Facilitating data transfer and improving precision in capillary zone electrophoresis with migration indexes Lee, Thomas T.; Yeung, Edward S. Analytical Chemistry, Vol. 63, Issue 24 https://doi.org/10.1021/ac00024a007	journal	December 1991
DNA sequencing by capillary electrophoresis with replaceable linear polyacrylamide and laser-induced fluorescence detection Ruiz-Martinez, Marie C.; Berka, Jan.; Belenkii, Alexei. Analytical Chemistry, Vol. 65, Issue 20 https://doi.org/10.1021/ac00068a023	journal	October 1993
Multiple Sheath-Flow Gel Capillary-Array Electrophoresis for Multicolor Fluorescent DNA Detection Takahashi, Satoshi.; Murakami, Katsuhiko.; Anazawa, Takashi. Analytical Chemistry, Vol. 66, Issue 7 https://doi.org/10.1021/ac00079a016	journal	April 1994
DNA sequencing using capillary array electrophoresis Huang, Xiaohua C.; Quesada, Mark A.; Mathies, Richard A. Analytical Chemistry, Vol. 64, Issue 18 https://doi.org/10.1021/ac00042a021	journal	September 1992
High-performance electrophoresis Hjertén, Stellan Journal of Chromatography A, Vol. 347 https://doi.org/10.1016/S0021-9673(01)95485-8	journal	January 1985
Optimization of capillary electrophoretic separation of DNA fragments based on polymer filled capillaries McGregor, David A.; Yeung, Edward S. Journal of Chromatography A, Vol. 652, Issue 1 https://doi.org/10.1016/0021-9673(93)80646-P	journal	October 1993
Capillary array electrophoresis using laser-excited confocal fluorescence detection Huang, Xiaohua C.; Quesada, Mark A.; Mathies, Richard A. Analytical Chemistry, Vol. 64, Issue 8 https://doi.org/10.1021/ac00032a025	journal	April 1992
Laser fluorescence detector for capillary electrophoresis Yeung, Edward S.; Wang, Poguang; Li, Wenni Journal of Chromatography A, Vol. 608, Issue 1-2 https://doi.org/10.1016/0021-9673(92)87107-J	journal	September 1992
Laser Fluorometric Detection in Liquid Chromatography Young, Edward S.; Sepaniak, Michael J. Analytical Chemistry, Vol. 52, Issue 13, p. 1465A-1481A https://doi.org/10.1021/ac50063a804	journal	November 1980
Three DNA sequencing methods using capillary gel electrophoresis and laser-induced fluorescence Swerdlow, Harold.; Zhang, Jian Zhong.; Chen, Da Yong. Analytical Chemistry, Vol. 63, Issue 24 https://doi.org/10.1021/ac00024a006	journal	December 1991
Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis. Cohen, A. S.; Najarian, D. R.; Paulus, A. Proceedings of the National Academy of Sciences, Vol. 85, Issue 24 https://doi.org/10.1073/pnas.85.24.9660	journal	December 1988
Lane distortions in gel electrophoresis patterns Starita-Geribaldi, Mireille; Houri, André; Sudaka, Pierre Electrophoresis, Vol. 14, Issue 1 https://doi.org/10.1002/elps.11501401121	journal	January 1993
High-resolution capillary electrophoretic analysis of DNA in free solution Nathakarnkitkool, Suraphol; Oefner, Peter J.; Bartsch, Georg Electrophoresis, Vol. 13, Issue 1 https://doi.org/10.1002/elps.1150130105	journal	January 1992
DNA sequencing with chain-terminating inhibitors Sanger, F.; Nicklen, S.; Coulson, A. R. Proceedings of the National Academy of Sciences, Vol. 74, Issue 12 https://doi.org/10.1073/pnas.74.12.5463	journal	December 1977
Multiwavelength fluorescence detection for DNA sequencing using capillary electrophoresis Karger, Achim E.; Harris, Joel M.; Gesteland, Raymond F. Nucleic Acids Research, Vol. 19, Issue 18 https://doi.org/10.1093/nar/19.18.4955	journal	January 1991
Temperature programming in capillary zone electrophoresis Whang, Chen Wen.; Yeung, Edward S. Analytical Chemistry, Vol. 64, Issue 5 https://doi.org/10.1021/ac00029a010	journal	March 1992
Axial-beam laser-excited fluorescence detection in capillary electrophoresis Taylor, John A.; Yeung, Edward S. Analytical Chemistry, Vol. 64, Issue 15 https://doi.org/10.1021/ac00039a020	journal	August 1992
Multiplexed fluorescence detector for capillary electrophoresis using axial optical fiber illumination Taylor, John A.; Yeung, Edward A. Analytical Chemistry, Vol. 65, Issue 7 https://doi.org/10.1021/ac00055a021	journal	April 1993
High-performance charge transfer device detectors Sweedler, Jonathan V.; Bilhorn, Robert B.; Epperson, Patrick M. Analytical Chemistry, Vol. 60, Issue 4 https://doi.org/10.1021/ac00155a002	journal	February 1988
Applications of charge transfer devices in spectroscopy Epperson, Patrick M.; Sweedler, Jonathan V.; Bilhorn, Robert B. Analytical Chemistry, Vol. 60, Issue 5 https://doi.org/10.1021/ac00156a001	journal	March 1988

Similar Records

Multiplexed capillary electrophoresis system

Patent · Mon Dec 09 23:00:00 EST 1996 · OSTI ID:415746

Multiplexed capillary electrophoresis system

Patent · Tue Apr 21 00:00:00 EDT 1998 · OSTI ID:644379

Multiplexed capillary electrophoresis system

Patent · Tue Apr 21 00:00:00 EDT 1998 · OSTI ID:871480

Related Subjects

/204/
4-label
array
base
base calling
binary
binary poly
calling
capillaries
capillary
capillary electrophoresis
channels
charge-injection
charge-injection device
coupled
device
dna
dna sequencing
electrophoresis
electrophoretic
electrophoretic separation
electrophoretic separations
emission
emission channels
ethyleneoxide
excitation
excitation geometry
filters
fluorescence
fluorescence emission
geometry
identification
imaged
improved
injection device
interior
long-pass
long-pass filters
matrix
multiplexed
multiplexed capillary
nucleotide
nucleotide identification
optical
optical excitation
optically
optically coupled
pass filter
pass filters
pixel
poly
provides
reactions
sanger-type
separations
sequencing
sequencing reaction
sequencing reactions
side-entry
side-entry optical
split
split fluorescence

Multiplexed capillary electrophoresis system

Citation Formats

References (41)

Similar Records

Related Subjects