Multiplexed capillary electrophoresis system
Patent
·
OSTI ID:644379
The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification (``base calling``) is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations. 19 figs.
- Research Organization:
- Iowa State University
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- W-7405-ENG-82
- Assignee:
- Iowa State Univ. Research Foundation, Ames, IA (United States)
- Patent Number(s):
- US 5,741,411/A/
- Application Number:
- PAN: 8-665,800
- OSTI ID:
- 644379
- Country of Publication:
- United States
- Language:
- English
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