Respiration-linked proton flux in Wolinella succinogenes during reduction of N-oxides
Journal Article
·
· Arch. Biochem. Biophys.; (United States)
Formate uncoupled proton translocation in formate-grown Wolinella succinogenes cells supplied with N-oxides as terminal electron acceptors. In suspensions containing KSCN (but not valinomycin), H2 supported proton translocation when NO3-, NO2-, and NO were provided as oxidants. H+/N-oxide ratios were 4.77 for NO3-, 2.49 for NO2-, and 1.75 for NO. KSCN inhibits N2O reduction thus precluding use of N2O as oxidant. Repeated exposure of cells to NO inhibited their ability to translocated protons with NO as oxidant but only slightly diminished and did not eliminate their capacity for NO3(-)- or NO2(-)-dependent proton flux. Substituting reduced benzyl viologen for H2 and measuring proton uptake provided results consistent with an extramembranal location for the N- oxide reductases. The uncoupler, carbonyl cyanide m-chlorophenylhydrazone, collapsed proton gradients, permitted uptake of 2 mol H+/mol NO3- or NO2-, but unaccountably inhibited NO3- reduction by 50% while leaving H+ uptake stoichiometry of the cells unaffected.
- Research Organization:
- Univ. of Georgia, Athens
- OSTI ID:
- 5632260
- Journal Information:
- Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 2; ISSN ABBIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BARYONS
BIOLOGICAL EFFECTS
CARBONYLS
CARBOXYLIC ACID SALTS
CHALCOGENIDES
CHEMICAL REACTIONS
CYANIDES
ELECTRONS
ELEMENTARY PARTICLES
ELEMENTS
FERMIONS
FORMATES
HADRONS
HYDRAZONES
HYDROGEN
INHIBITION
LEPTONS
MICROORGANISMS
NITROGEN COMPOUNDS
NITROGEN OXIDES
NONMETALS
NUCLEONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PROTONS
REDUCTION
RESPIRATION
TRANSLOCATION
VALENCE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BARYONS
BIOLOGICAL EFFECTS
CARBONYLS
CARBOXYLIC ACID SALTS
CHALCOGENIDES
CHEMICAL REACTIONS
CYANIDES
ELECTRONS
ELEMENTARY PARTICLES
ELEMENTS
FERMIONS
FORMATES
HADRONS
HYDRAZONES
HYDROGEN
INHIBITION
LEPTONS
MICROORGANISMS
NITROGEN COMPOUNDS
NITROGEN OXIDES
NONMETALS
NUCLEONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PROTONS
REDUCTION
RESPIRATION
TRANSLOCATION
VALENCE