Energy coupling to nitrite respiration in the sulfate reducing bacterium Desulfovibrio gigas
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:5997847
By use of a membrane fraction prepared from Desulfovibrio gigas grown in a lactate-sulfate medium, synthesis of ATP was demonstrated to be coupled to the oxidation of molecular hydrogen and reduction of either nitrite or hydroxylamine. This phosphorylation was uncoupled from electron transport by pentachlorophenol, methyl viologen, and gramicidin, but not by oligomycin. The extrusion of protons from the cells was shown to be coupled to the hydrogen-nitrite respiratory system, and, assuming the localization of nitrite reductase on the outer side of the plasma membrane, H/sup +//2e/sup -/ values of 2.0 +/- 0.3 were obtained. Energy coupling observed with this system appears to be due to electron transfer-coupled proton translocation rather than vectorial electron transfer associated with hydrogen oxidation. 29 references, 1 figure, 4 tables.
- Research Organization:
- Univ. of New Mexico, Albuquerque
- DOE Contract Number:
- AS09-79ER10499
- OSTI ID:
- 5997847
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 153:2; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Journal Article
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Wed Jun 10 00:00:00 EDT 1981
· J. Biol. Chem.; (United States)
·
OSTI ID:6503554
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Journal Article
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Wed Jul 01 00:00:00 EDT 1981
· J. Bacteriol.; (United States)
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Journal Article
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Mon Nov 30 23:00:00 EST 1981
· J. Biol. Chem.; (United States)
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Related Subjects
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AROMATICS
ATP
AZINES
BACTERIA
BIOLOGICAL EFFECTS
BIPYRIDINES
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
COUPLING
DATA
DESULFOVIBRIO
DRUGS
ELECTRON TRANSFER
ENZYMES
EXPERIMENTAL DATA
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
HYDROXYLAMINE
INFORMATION
MEMBRANES
MICROORGANISMS
NITRITES
NITROGEN COMPOUNDS
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PHENOLS
PHOSPHORYLATION
PYRIDINES
RESPIRATION
SULFATE-REDUCING BACTERIA
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AROMATICS
ATP
AZINES
BACTERIA
BIOLOGICAL EFFECTS
BIPYRIDINES
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
COUPLING
DATA
DESULFOVIBRIO
DRUGS
ELECTRON TRANSFER
ENZYMES
EXPERIMENTAL DATA
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
HYDROXYLAMINE
INFORMATION
MEMBRANES
MICROORGANISMS
NITRITES
NITROGEN COMPOUNDS
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PHENOLS
PHOSPHORYLATION
PYRIDINES
RESPIRATION
SULFATE-REDUCING BACTERIA