Proton translocation associated with nitrite respiration in Desulfovibrio desulfuricans

Journal Article · Wed Jun 10 04:00:00 EDT 1981 · J. Biol. Chem.; (United States)

OSTI ID:6503554

Steenkamp, D J; Peck, Jr, H D

Proton translocation by Desulfovibrio desulfuricans cells, cultured anaerobically with Nitrate as terminal oxidant, was studied by the oxidant-pulse method. Nitrate-grown D. desulfuricans translocated protons rapidly and reproducibly with hydrogen as reductant and nitrite as electron acceptor. H/sup +//2e/sup -/ ratios were typically in the range 1.8-2.2. Proton translocation following pulses of nitrite was also observed with endogenous substrate in freshly harvested cells and with lactate or formate as electron donors in starved cells. Problems in the determination of H/sup +//2e/sup -/ ratios when endogenous substrate, formate, or lactate was the electron donor are discussed. Evidence is presented for the location of formate dehydrogenase, hydrogenase, and nitrite reductase on the periplasmic and for lactate dehydrogenase on the cytoplasmic side of the cytoplasmic membrane.

• Research Organization: Univ. of Georgia, Athens
• DOE Contract Number: AS09-79ER10499
• OSTI ID: 6503554
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 256:11; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED-PARTICLE TRANSPORT
CHEMICAL REACTIONS
DENITRIFICATION
ENZYME ACTIVITY
MEMBRANES
MICROORGANISMS
NITRITES
NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PROTON TRANSPORT
RADIATION TRANSPORT
RESPIRATION