Proton translocation coupled to dimethyl sulfoxide reduction in anaerobically grown Escherichia coli HB101

Journal Article · Mon Jul 01 04:00:00 EDT 1985 · J. Bacteriol.; (United States)

OSTI ID:5148338

Bilous, P T; Weiner, J H

Proton translocation coupled to dimethyl sulfoxide (DMSO) reduction was examined in Escherichia coli HB101 grown anaerobically on glycerol and DMSO. Rapid acidification of the medium was observed when an anaerobic suspension of cells, preincubated with glycerol, was pulsed with DMSO, methionine sulfoxide, nitrate, or trimethylamine N-oxide. The DMSO-induced acidification was sensitive to the uncoupler carbonyl cyanide p-trifluoromethoxyphenylhydrazone (60 microM) and was inhibited by the quinone analog 2-n-heptyl-4-hydroxy-quinoline-N-oxide (5.6 microM). Neither sodium azide nor potassium cyanide inhibited the DMSO response. An apparent----H+/2e- ratio of 2.9 was obtained for DMSO reduction with glycerol as the reductant. Formate and H2(g), but not lactate, could serve as alternate electron donors for DMSO reduction. Cells grown anaerobically on glycerol and fumarate displayed a similar response to pulses of DMSO, methionine sulfoxide, nitrate, and trimethylamine N-oxide with either glycerol or H2(g) as the electron donor. However, fumarate pulses did not result in acidification of the suspension medium. Proton translocation coupled to DMSO reduction was also demonstrated in membrane vesicles by fluorescence quenching. The addition of DMSO to hydrogen-saturated everted membrane vesicles resulted in a carbonyl cyanide p-trifluoromethoxyphenyl-hydrazone-sensitive fluorescence quenching of quinacrine dihydrochloride. The data indicate that reduction of DMSO by E. coli is catalyzed by an anaerobic electron transport chain, resulting in the formation of a proton motive force.

• Research Organization: Univ. of Alberta, Edmonton, Canada
• OSTI ID: 5148338
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACIDIFICATION
ALCOHOLS
ALKALI METALS
AMINO ACIDS
ANAEROBIC CONDITIONS
AROMATICS
AZAARENES
AZIDES
AZINES
BACTERIA
BARYONS
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CULTURE MEDIA
CYANIDES
DMSO
DRUGS
ELEMENTARY PARTICLES
ELEMENTS
ESCHERICHIA COLI
FERMIONS
FORMATES
GLYCEROL
GROWTH
HADRONS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
KINETICS
LIPOTROPIC FACTORS
METABOLISM
METALS
METHIONINE
MICROORGANISMS
NITRATES
NITROGEN COMPOUNDS
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
POTASSIUM
PROTONS
PYRIDINES
QUINOLINES
REACTION KINETICS
REDOX REACTIONS
SODIUM
SULFOXIDES
TRANSLOCATION