Vanadate and dicyclohexylcarbodiimide insensitive proton extrusion from oxygen pulsed cells of the cyanobacterium Anacystis nidulans
Oxygen pulses applied to dark anaerobic suspensions of Anacystis nidulans provoked immediate acidification of the external medium. The reaction was inhibited only 75% by dicyclohexylcarbodiimide and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole at concentrations which completely arrested all oxidative phosphorylation. Carbonyl cyanide m-chlorophenylhydrazone eliminated the acidification of oxygen pulsed cell suspensions while ortho-vanadate and diethylstilbestrol had no effect. No lag occurred between the onset of respiration and proton extrusion. H/sup +//O ratios were 4.1 +/- 0.5 in the absence, and 1.9 +/- 0.4 in the presence, of dicyclohexylcarbodiimide. These results are consistent with a recently described proton-translocating aa/sub 3/-type cytochrome c oxidase (H/sup +//O = 1.6 +/- 0.4) in the cell membrane of A. nidulans. 21 references, 4 figures, 1 table.
- Research Organization:
- Univ. of Vienna, Austria
- OSTI ID:
- 5934875
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 123:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL MEMBRANES
BIOCHEMISTRY
IMIDES
BIOLOGICAL EFFECTS
VANADATES
CYANOBACTERIA
OXYGEN
PROTONS
RESPIRATION
VISIBLE RADIATION
BARYONS
CELL CONSTITUENTS
CHEMISTRY
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
ELEMENTS
FERMIONS
HADRONS
MEMBRANES
MICROORGANISMS
NONMETALS
NUCLEONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
RADIATIONS
TRANSITION ELEMENT COMPOUNDS
VANADIUM COMPOUNDS
550200* - Biochemistry