Vanadate and dicyclohexylcarbodiimide insensitive proton extrusion from oxygen pulsed cells of the cyanobacterium Anacystis nidulans

Journal Article · Thu Aug 30 04:00:00 EDT 1984 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(84)90421-2· OSTI ID:5934875

Nitschmann, W H; Peschek, G A

Oxygen pulses applied to dark anaerobic suspensions of Anacystis nidulans provoked immediate acidification of the external medium. The reaction was inhibited only 75% by dicyclohexylcarbodiimide and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole at concentrations which completely arrested all oxidative phosphorylation. Carbonyl cyanide m-chlorophenylhydrazone eliminated the acidification of oxygen pulsed cell suspensions while ortho-vanadate and diethylstilbestrol had no effect. No lag occurred between the onset of respiration and proton extrusion. H/sup +//O ratios were 4.1 +/- 0.5 in the absence, and 1.9 +/- 0.4 in the presence, of dicyclohexylcarbodiimide. These results are consistent with a recently described proton-translocating aa/sub 3/-type cytochrome c oxidase (H/sup +//O = 1.6 +/- 0.4) in the cell membrane of A. nidulans. 21 references, 4 figures, 1 table.

• Research Organization: Univ. of Vienna, Austria
• OSTI ID: 5934875
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 123:1; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BARYONS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMISTRY
CYANOBACTERIA
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
ELEMENTS
FERMIONS
HADRONS
IMIDES
MEMBRANES
MICROORGANISMS
NONMETALS
NUCLEONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN
OXYGEN COMPOUNDS
PROTONS
RADIATIONS
RESPIRATION
TRANSITION ELEMENT COMPOUNDS
VANADATES
VANADIUM COMPOUNDS
VISIBLE RADIATION