Two histidine-rich HRGPS from Zea mays
Conference
·
· Plant Physiology, Supplement; (USA)
OSTI ID:5629019
- Michigan State Univ., East Lansing (USA)
We used intact cell elution to isolate two maize HRGPs that co-chromatographed, but resolved after HF-deglycosylation, into two bands (M{sub r} 68 70 kD) on SDS PAGE. They were rich in His, thus designated HHRGPs, and contained 65% carbohydrate occurring as O-arabinosylated Hyp (63 mole%) and Gal (37%) possibly occurring as polygalactose on Thr or Ser. HHRGP did not react with Yariv artificial antigen, or agglutinate rabbit reticulocytes, implying that they are not arabinogalactan proteins or lectins. Furthermore, they were not in a polyproline-II conformation, judging by CD spectra, although TEM showed tham as extended rods. Quantitative ELISAs showed that antibodies raised against deglycosylated HHRGP crossreacted with deglycosylated tomato extensins P1 P2, suggesting possible homology between HHRGP and tomato extensin. Chymotryptic digestion of deglycosylated HHRGP gave a peptide map with 12 peptides rich in His, Ala and Hyp and confirming that both protein bands resolving on SDS-PAGE were HHRGPs.
- OSTI ID:
- 5629019
- Report Number(s):
- CONF-9007196--
- Conference Information:
- Journal Name: Plant Physiology, Supplement; (USA) Journal Volume: 93:1
- Country of Publication:
- United States
- Language:
- English
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