Structure of the threonine-rich extensin from Zea mays

Journal Article · Thu Feb 01 04:00:00 EST 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.92.2.316· OSTI ID:5859329

Kieliszewski, M J; Leykam, J F; Lamport, D T.A. ^[1]

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1. Michigan State Univ., East Lansing (USA)

Chymotryptic digestion of a threonine-rich hydroxyproline-rich glycoprotein (THRGP) purified from the cell surface of a Zea mays cell suspension culture gave a peptide map dominated by the hexadecapeptide TC5: Thr-Hyp-Ser-Hyp-Lys-Pro-Hyp-Thr-Pro-Lys-Pro-Thr-Hyp-Thr-Thr, in which the repetitive motif Ser-Hyp-Lys-Pro-Hyp-Thr-Pro-Lys is homologous with the dominant decamer of P1-type dicot extensins: Ser-Hyp-Hyp-Hyp-Hyp-Thr-Hyp-Val-Tyr-Lys, modified by a Lys for Hyp substitution at residue 3, a Val-Tyr deletion at residues 8 and 9, and incomplete posttranslational modification of proline residues. One of the minor peptides (TC1) contained the 8-residue sequence: Thr-Hyp-Ser-Hyp-Hyp-Hyp-Hyp-Tyr corresponding to the C-terminal tail (judging from the recently isolated maize cDNA clone MC56) which is homologous with the major repetitive motif of the P3 class of dicot extensins. Direct peptide sequencing defined potential glycosylated regions on the THRGP corresponding to clone MC56 and showing that glycosylated and nonglycosylated domains alternate with high regularity. In a quantitative enzyme-linked immunosorbent assay, these antibodies cross-reacted 20% with tomato P1 extensin, and 18% with anhydrous hydrogen fluoride-deglycosylated P1. These results, together with other previously published data, show that maize THRGP is homologous with the dicot P1 extensins and, as such, is the first extensin isolated from a graminaceous monocot.

• OSTI ID: 5859329
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 92:2; ISSN PLPHA; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL WALL
CEREALS
DNA SEQUENCING
GLYCOPROTEINS
GRASS
HYDROXY ACIDS
LILIOPSIDA
MAGNOLIOPHYTA
MAIZE
NATIONAL ORGANIZATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
PHYSIOLOGY
PLANT CELLS
PLANTS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
THREONINE
US DOE
US ORGANIZATIONS