Extensins in graminaceous monocots and dicots compared

Conference · Wed Apr 01 04:00:00 EST 1987 · Plant Physiol., Suppl.; (United States)

OSTI ID:6080895

Kieliszewski, M; Lamport, D T.A.

Is the cellulose-extensin warp-weft model of primary cell wall organization generally applicable, or restricted to dicots. Although wall-bound hydroxyproline is usually a quantitative measure of extensin content, it is not definitive at low hyp levels typical of graminaceous monocots. Therefore the authors searched for putative soluble extensins ionically-bound to the cell wall of maize cell suspension cultures. Fractionation of AlCl/sub 3/ eluates on cellex-P, Cellex-E, and Superose-6 gave two HRGPs CEV and CE1 which compositionally resembled extensins rather than the arabinogalactan HRGPs. CEV and CE1 did not react with Yariv's antigen, but did cross-react with antibodies raised against tomato extensin. A tryptic peptide map of CEV gave some major peptides enriched in hydroxyproline and proline residues, indicating peptide periodicity. TEM of the low-angle shadowed protein gave flexuous rodlike molecules of 80 nm contour length. This is the best evidence to date for extensin in monocots.

• Research Organization: Michigan State Univ., East Lansing
• OSTI ID: 6080895
• Report Number(s): CONF-8707108-
• Conference Information: Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 83:4
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANTIGEN-ANTIBODY REACTIONS
AZOLES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL WALL
CEREALS
ELECTRON MICROSCOPY
FRACTIONATION
GRASS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
HYDROXYPROLINE
MAIZE
MICROSCOPY
MOLECULAR STRUCTURE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PYRROLES
PYRROLIDINES
SEPARATION PROCESSES
TRANSMISSION ELECTRON MICROSCOPY