Methanogenesis from choline by a coculture of Desulfovibrio sp. and Methanosarcina barkeri
A sulfate-reducing vibrio was isolated from a methanogenic enrichment with choline as the sole added organic substrate. This oganism was identified as a member of the genus Desulfovibrio and was designated Desulfovibrio strain G1. In a defined medium devoid of sulfate, a pure culture of Desulfovibrio strain G1 fermented choline to trimethylamine, acetate, and ethanol. In the presence of sulfate, more acetate and less ethanol were formed from choline than in the absence of sulfate. When grown in a medium containing sulfate, a coculture of Desulfovibrio strain G1 and Methanosarcina barkeri strain Fusaro degraded chloline almost completely to methane, ammonia, and hydrogen sulfide and presumably to carbon dioxide. Methanogenesis occurred in two distinct phases separated by a lag of about 6 days. During the first phase of methanogenesis choline was completely converted to trimethylamine, acetate, hydrogen sulfide, and traces of ethanol by the desulfovibrio.M. barkeri fermented trimethylamine to methane, ammonia, and presumably carbon dioxide via dimethyl- and methylamine as intermediates. Simultaneously, about 60% of the acetate expected was metabolized. In the second phase of methanogenesis, the residual acetate was almost completely catabolized.
- Research Organization:
- Institute for Mikrobiologie der Universitat Gottingen, Federal Republic of Germany
- OSTI ID:
- 5627787
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 45:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMINES
FERMENTATION
CHOLINE
BIODEGRADATION
METHANE
BIOSYNTHESIS
ANAEROBIC CONDITIONS
CELL CULTURES
DESULFOVIBRIO
METHANOGENIC BACTERIA
SULFATES
ALCOHOLS
ALKANES
AMMONIUM COMPOUNDS
BACTERIA
BIOCONVERSION
CHEMICAL REACTIONS
DECOMPOSITION
DRUGS
HYDROCARBONS
HYDROXY COMPOUNDS
LIPOTROPIC FACTORS
MICROORGANISMS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
QUATERNARY COMPOUNDS
SULFUR COMPOUNDS
SYNTHESIS
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
090122 - Hydrocarbon Fuels- Preparation from Wastes or Biomass- (1976-1989)