Enzyme-entrapping behaviors in alginate fibers and their papers

Journal Article · Thu Jan 01 00:00:00 EST 1987 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260300316· OSTI ID:5625473

Kobayashi, Y; Matsuo, R; Ohya, T; Yokoi, N

Enzyme immobilization in the form of fiber and paper was easily achieved by wet spinning of aqueous admixture of sodium alginate and enzymes into divalent metallic ion solution as a coagulating bath, followed by paper making of resultant shortly cut fibers. Entrapment yields of enzymes used, e.g., glucoamylase, cyclodextrin glucanotransferase, endo-polygalacturonase, and protease, were always higher in calcium alginate fibers and their papers than those in corresponding beads. It was found that the yields increased with an increase of the discharge rate through the spinning nozzle because the higher discharge rate could provide more highly oriented metal-chelate linear polymer molecules along the fiber axis for preventing leakage of entrapped enzymes. Divalent metallic ions affected greatly the entrapment of glucoamylase in alginate fibers, the order of which followed rougly the ionotropic series of Thiele. Entrapment of glucoamylase in bicomponent systems comprising alginate and other water-soluble polymers was also investigated. (Refs. 41).

• Research Organization: Government Industrial Research Institute, Shikoku 2-3-3, Hananomiya-cho, Takamatsu, 761, Japan.; Nishiharu Factory, Amano Pharmaceutical Co. Ltd., Nishiharu, Nishikasugai-Gun, Aichi Prefecture, 481, Japan
• OSTI ID: 5625473
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 30:3
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
AMYLASE
VITRIFICATION
GLYCOSYL TRANSFERASES
IMMOBILIZED ENZYMES
PEPTIDE HYDROLASES
ALGINATES
CALCIUM
CATALYST SUPPORTS
FIBERS
IONS
METALS
PAPER
ALKALINE EARTH METALS
CHARGED PARTICLES
ELEMENTS
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
O-GLYCOSYL HYDROLASES
TRANSFERASES
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)