Structurally stable gel bead containing entrapped enzyme and method for manufacture thereof
This research provides a structurally stable gel bead containing an entrapped enzyme and a method for its manufacture. The enzyme is covalently cross-linked to gelatin in the presence of glutaraldehyde prior to the formation of the gel bead, to prevent leakage of the enzyme. Propylene glycol alginate is then added to the mixture. Once the gel beads are formed, they are then soaked in glutaraldehyde, which imparts structural stability to the gel beads. This method can be used with many types of enzymes, such as proteases, carbohydrases, proteases, ligases, isomerases, oxidoreductases, and specialty enzymes. These and other enzymes can be immobilized in the gel beads and utilized in a number of enzymatic processes. Exogenously added ions are not required to maintain the structural stability of these gel beads. 7 figs.
- Research Organization:
- Lockheed Martin Energy Syst Inc
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- AC05-84OR21400
- Assignee:
- Lockheed Martin Energy Research Systems, Inc., Oak Ridge, TN (United States)
- Patent Number(s):
- US 5,846,762/A/
- Application Number:
- PAN: 8-489,304
- OSTI ID:
- 321193
- Country of Publication:
- United States
- Language:
- English
Similar Records
Gel bead composition for metal adsorption
Gel bead composition for metal adsorption