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Structurally stable gel bead containing entrapped enzyme and method for manufacture thereof

Patent ·
OSTI ID:872026
 [1]
  1. Oak Ridge, TN
+ Show Author Affiliations

A structurally stable gel bead containing an entrapped enzyme and a method for its manufacture. The enzyme is covalently cross-linked to gelatin in the presence of glutaraldehyde prior to the formation of the gel bead, to prevent leakage of the enzyme. Propylene glycol alginate is then added to the mixture. Once the gel beads are formed, they are then soaked in glutaraldehyde, which imparts structural stability to the gel beads. This method can be used with many types of enzymes, such as proteases, carbohydrases, proteases, ligases, isomerases, oxidoreductases, and specialty enzymes. These and other enzymes can be immobilized in the gel beads and utilized in a number of enzymatic processes. Exogenously added ions are not required to maintain the structural stability of these gel beads.

Research Organization:
LOCKHEED MARTIN ENRGY SYST INC
DOE Contract Number:
AC05-84OR21400
Assignee:
Lockheed Martin Energy Research Systems, Inc. (Oak Ridge, TN)
Patent Number(s):
US 5846762
OSTI ID:
872026
Country of Publication:
United States
Language:
English

References (9)

β-Glucosidase: microbial production and effect on enzymatic hydrolysis of cellulose journal February 1977
Fungal and other β-d-glucosidases — Their properties and applications journal March 1982
Properties of native and immobilised preparations of β-D-glucosidase from Aspergillus niger journal January 1982
Hydrolysis of cellobiose by immobilized β-glucosidase entrapped in maintenance-free gel spheres journal March 1991
Enzymatic hydrolysis of cellulose to glucose lung immobilized ?-glucosidase journal March 1981
Use of Immobilized β-Glucosidase in the Hydrolysis of Cellulose book July 1993
Immobilization of Aspergillus beta-glucosidase on chitosan. journal January 1978
Properties and application of immobilized ?-D-glucosidase coentrapped withZymomonas mobilis in calcium alginate journal October 1983
Cellobiose hydrolysis by glutaraldehyde- treated β-glucosidase entrapped in propylene glycol alginate/bone gelatin spheres: Scientific note journal March 1992

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Related Subjects

/435/264/427/428/
added
alginate
bead
bead containing
beads
carbohydrases
containing
containing entrapped
covalently
cross-linked
entrapped
entrapped enzyme
enzymatic
enzyme
enzymes
exogenously
formation
formed
gelatin
glutaraldehyde
glycol
glycol alginate
immobilized
imparts
isomerases
leakage
ligases
maintain
manufacture
method
mixture
oxidoreductases
presence
prevent
prevent leakage
prior
processes
propylene
propylene glycol
proteases
required
soaked
specialty
stability
stable
structural
structural stability
structurally
structurally stable
types
utilized