Comparison of /sup 99m/Tc-DTPA and /sup 113m/In-DTPA aerosol clearances in humans. Effects of smoking, hyperinflation, and in vitro oxidation

Journal Article · Sun Nov 01 04:00:00 EST 1987 · Am. Rev. Respir. Dis.; (United States)

DOI:https://doi.org/10.1164/ajrccm/136.5.1112· OSTI ID:5620284

Nolop, K B; Maxwell, D L; Fleming, J S; Braude, S; Hughes, J M; Royston, D

As an index of permeability of the alveolar epithelium, the clearance of an inhaled aerosol of /sup 99m/Tc-DTPA is increased in several disease states. However, the usefulness of the test to assess the severity of disease is limited because healthy smokers also have abnormally rapid rates of clearance. Because the stability of the /sup 99m/Tc-DTPA bond might be a contributory factor, we tested the affinity of /sup 99m/Tc for DTPA in vitro, and in groups of healthy smokers (n = 13) and nonsmokers (n = 7) we measured the clearances of /sup 99m/Tc-DTPA and /sup 113m/In-DTPA, which have a similar molecular shape and charge. In vitro, sodium hypochlorite or hydrogen peroxide released as much as 98% of free /sup 99m/Tc from the /sup 99m/Tc-DTPA complex. When incubated with human neutrophils stimulated with phorbol myristate acetate, between 4 and 7% of free /sup 99m/Tc-DTPA was released after 30 min, and 12% was released after 60 min. In vivo, the clearances of both /sup 99m/Tc-DTPA and /sup 113m/In-DTPA in the smokers (n = 13) were faster than in the nonsmokers (n = 7) (p less than 0.05). Within the smokers, the mean /sup 99m/Tc-DTPA clearance (T1/2 25 +/- 4 min) was faster than the mean /sup 113m/In-DTPA clearance (34 +/- 6 min), (p less than 0.05). For nonsmokers, the difference was smaller (T1/2 /sup 99m/Tc-DTPA, 56 +/- 6; T1/2 /sup 113m/In-DTPA, 62 +/- 6) and not significant. During hyperinflation, smokers (n = 8) and nonsmokers (n = 8) both demonstrated an increase in /sup 113m/In-DTPA clearance.

• Research Organization: Royal Postgraduate Medical School, London, England
• OSTI ID: 5620284
• Journal Information: Am. Rev. Respir. Dis.; (United States), Journal Name: Am. Rev. Respir. Dis.; (United States) Vol. 136:5; ISSN ARDSB
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
AMINO ACIDS
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CARCINOGENS
CHELATING AGENTS
CHEMICAL REACTIONS
CLEARANCE
COLLOIDS
COMPARATIVE EVALUATIONS
DISEASES
DISPERSIONS
DRUGS
DTPA
EPITHELIUM
ESTERS
HOURS LIVING RADIOISOTOPES
IN VITRO
INDIUM 113
INDIUM ISOTOPES
INHALATION
INTAKE
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
MATERIALS
METABOLISM
NEUTROPHILS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PERMEABILITY
PHORBOL ESTERS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
REDOX REACTIONS
RESIDUES
RESPIRATORY SYSTEM DISEASES
SMOKES
SOLS
STABLE ISOTOPES
TECHNETIUM 99
TECHNETIUM ISOTOPES
TISSUES
TOBACCO SMOKES
YEARS LIVING RADIOISOTOPES