Direct measurement of brain glucose concentrations in humans by sup 13 C NMR spectroscopy

Journal Article · Sat Feb 01 04:00:00 EST 1992 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.89.3.1109· OSTI ID:5617900

Gruetter, R; Novotny, E J; Boulware, S D; Rothman, D L; Mason, G F; Shulman, G I; Shulan, R G; Tamborlane, W V ^[1]

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1. Yale Univ., New Haven, CT (United States)

Glucose is the main fuel for energy metabolism in the normal human brain. It is generally assumed that glucose transport into the brain is not rate-limiting for metabolism. Since brain glucose concentrations cannot be determined directly by radiotracer techniques, the authors used {sup 13}C NMR spectroscopy after infusing enriched D-(1-{sup 13}C)glucose to measure brain glucose concentrations at euglycemia and at hyperglycemia in six healthy children. Brain glucose concentrations averaged 1.0 {plus minus} 0.1 {mu}mol/ml at euglycemia and 1.8-2.7 {mu}mol/ml at hyperglycemia. Michaelis-Menten parameters of transport were calculated from the relationship between plasma and brain glucose concentrations. The brain glucose concentrations and transport constants are consistent with transport not being rate-limiting for resting brain metabolism at plasma levels >3 mM.

• OSTI ID: 5617900
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 89:3; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGE GROUPS
ALDEHYDES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY
BODY FLUIDS
BRAIN
CARBOHYDRATES
CARBON 13
CARBON ISOTOPES
CENTRAL NERVOUS SYSTEM
CHILDREN
DOSE-RESPONSE RELATIONSHIPS
DYNAMIC FUNCTION STUDIES
EVEN-ODD NUCLEI
GLUCOSE
HEXOSES
INFUSION
INTAKE
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MATERIALS
MONOSACCHARIDES
NERVOUS SYSTEM
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ORGANIC COMPOUNDS
ORGANS
RESONANCE
SACCHARIDES
STABLE ISOTOPES